Abstract

The paper is devoted to characterization of BEA and MFI zeolite samples synthesized in alkaline and fluoride media. Using a number of synthesized and commercial samples, enzyme adsorption isotherms were recorded, specifically for Aspergillus oryzae β-galactosidase. The highest adsorption capacity (32–33 mg/g) was achieved on fine-crystalline (0.2–0.6 μm) MFI samples. The fine-crystalline BEA samples (0.3–1 and ˂0.1 μm) exhibited maximal adsorption capacities of 18 and 26 mg/g, respectively. The coarse-crystalline BEA and MFI zeolites prepared by fluoride synthesis reached a markedly lower enzyme adsorption capacity, not exceeding 6 mg/g. The NMR spectroscopy data indicate that the low adsorption capacity of the zeolites synthesized in fluoride media was not only owing to their crystal size but also to the low content of hydroxyl groups on their surface.

中文翻译：

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沸石合成条件及结构特性对β-半乳糖苷酶吸附的影响

摘要

本文致力于表征在碱性和氟化物介质中合成的 BEA 和 MFI 沸石样品。使用大量合成和商业样品，记录酶吸附等温线，特别是米曲霉β-半乳糖苷酶。在细晶（0.2-0.6 μm）MFI 样品上实现了最高吸附容量（32-33 mg/g）。细晶 BEA 样品（0.3-1 和 ˂0.1 μm）分别表现出 18 和 26 mg/g 的最大吸附容量。通过氟化物合成制备的粗晶BEA和MFI沸石达到显着较低的酶吸附能力，不超过6 mg/g。NMR光谱数据表明，在氟化物介质中合成的沸石的低吸附能力不仅是由于它们的晶体尺寸，而且是由于它们表面的羟基含量低。