Organic fraction of municipal solid waste (OFMSW) has high volatile solid and moisture content, which is mainly treated via anaerobic digestion (AD). Since the composition of OFMSW is complicated and inconstant, it is difficult to maintain the AD process stable and effective. In this study, a novel two-stage AD system, including a tank-type acidogenic phase and a fixed-bed methanogenic phase, was implemented for OFMSW treatment to demonstrate the feasibility of the two-stage configuration. Results showed that 84% of potential CH₄ yield was achieved and the methane content reached 77.83% on average in methanogenic phase. The acetoclastic methanogens, Methanosaeta and Methanosarcina, which can participate in direct interspecies electron transfer, were the dominant genera in solid digestate and the biofilm. The hydrogenotrophic methanogens, Methanospirillum and Methanoculleus, accounted for high proportions in liquid digestate. Our study provides a novel approach to treat OFMSW with less water consumption, high stability and efficiency.

城市固体废物的有机部分（OFMSW）具有较高的挥发性固体和水分含量，主要通过厌氧消化（AD）处理。由于OFMSW的成分复杂且不稳定，因此难以保持AD过程稳定有效。在这项研究中，对OFMSW处理实施了一种新型的两级AD系统，包括罐式产酸相和固定床产甲烷相，以证明两级配置的可行性。结果表明，在产甲烷阶段，CH ₄潜在产率达到了84%，甲烷含量平均达到了77.83%。乙酸碎屑产甲烷菌Methanosaeta和Methanosarcina可参与直接种间电子转移的属，是固体消化物和生物膜中的优势属。氢营养产甲烷菌Methanospirillum和Methanoculleus占液体消化物中的高比例。我们的研究提供了一种新的方法来处理OFMSW，其耗水量少、稳定性和效率高。