The regenerative potential in response to wounding varies widely among species. Within the plant lineage, the liverwort Marchantia polymorpha displays an extraordinary regeneration capacity. However, its molecular pathways controlling the initial regeneration response are unknown. Here, we demonstrate that the Mp ERF15 transcription factor gene is instantly activated after wounding and is essential for gemmaling regeneration following tissue incision. MpERF15 operates both upstream and downstream of the MpCOI1 oxylipin receptor by controlling the expression of oxylipin biosynthesis genes. The resulting rise in the oxylipin dinor-12-oxo-phytodienoic acid (dn-OPDA) levels results in an increase in gemma cell number and apical notch organogenesis, generating highly disorganized and compact thalli. Our data pinpoint Mp ERF15 as a key factor activating an oxylipin biosynthesis amplification loop after wounding, which eventually results in reactivation of cell division and regeneration. We suggest that the genetic networks controlling oxylipin biosynthesis in response to wounding might have been reshuffled over evolution.

中文翻译：

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伤口激活的 ERF15 转录因子通过激活 oxylipin 生物合成反馈回路来驱动多形金缕梅再生

对伤害做出反应的再生潜力因物种而异。在植物谱系中，地钱金盏花显示出非凡的再生能力。然而，其控制初始再生反应的分子途径尚不清楚。在这里，我们证明了 MpERF15转录因子基因在受伤后立即被激活，并且对于组织切口后的宝石再生至关重要。MpERF15 通过控制氧化脂生物合成基因的表达，在 MpCOI1 氧化脂受体的上游和下游起作用。由此产生的 oxylipin dinor-12-oxo-phytodienoic acid (dn-OPDA) 水平的升高导致胚细胞数量和顶端切口器官发生的增加，从而产生高度杂乱和紧凑的菌体。我们的数据精确定位 MpERF15作为受伤后激活氧磷脂生物合成放大环的关键因素，最终导致细胞分裂和再生的重新激活。我们认为控制氧化脂生物合成以响应伤害的遗传网络可能已经在进化过程中重新洗牌。