To determine whether oxidative stress and inflammation are associated with constipation by examining the expression of the main producers of reactive oxygen species, nicotinamide adenine dinucleotide phosphate (NADPH) oxidases, and pro-inflammatory cytokines in the colon of patients with chronic functional constipation. The colonic biopsies were collected from 32 patients with chronic functional constipation and 30 healthy subjects who underwent colonoscopy. Colonic mucosal histology was observed. Interleukin (IL)-1β, IL-6, IL-8 messenger RNA (mRNA), and 4 members of NADPH oxidase (NOX1, NOX2, DUOX2, and NOX4) protein and mRNA were assessed by immunohistochemistry, western blotting, and reverse transcription polymerase chain reaction. The tissues from both patients and healthy subjects showed normal histological structure without increase of inflammatory cells. NOX1 protein and mRNA levels were significantly increased compared to controls (P < .05). DUOX2 protein, but not mRNA, was increased by 2-fold compared to controls (P < .05). The levels of NOX2 and NOX4 protein and mRNA demonstrated no significant difference between patients and control subjects. The levels of IL-1β and IL-6 mRNA were significantly higher in constipation patients (P < .05), while IL-8 mRNA level was no different between the 2 groups. NADPH oxidase and pro-inflammatory cytokine might be involved in the pathogeneses of chronic functional constipation.

中文翻译：

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慢性功能性便秘患者结肠粘膜中 NOX1 和 DUOX2 表达增加。

通过检查慢性功能性便秘患者结肠中活性氧的主要产生者、烟酰胺腺嘌呤二核苷酸磷酸 (NADPH) 氧化酶和促炎细胞因子的表达，确定氧化应激和炎症是否与便秘相关。结肠活检取自 32 名慢性功能性便秘患者和 30 名接受结肠镜检查的健康受试者。观察结肠粘膜组织学。通过免疫组织化学、蛋白质印迹和逆转录评估白介素 (IL)-1β、IL-6、IL-8 信使 RNA (mRNA) 和 NADPH 氧化酶的 4 个成员（NOX1、NOX2、DUOX2 和 NOX4）蛋白和 mRNA聚合酶链反应。来自患者和健康受试者的组织显示出正常的组织学结构，没有炎症细胞的增加。与对照组相比，NOX1 蛋白和 mRNA 水平显着增加 (P < .05)。与对照相比，DUOX2 蛋白而非 mRNA 增加了 2 倍 (P < .05)。NOX2 和 NOX4 蛋白和 mRNA 的水平在患者和对照受试者之间没有显着差异。便秘患者的 IL-1β 和 IL-6 mRNA 水平显着升高 (P < .05)，而 IL-8 mRNA 水平在两组间无差异。NADPH氧化酶和促炎细胞因子可能参与慢性功能性便秘的发病机制。与对照组相比增加了 2 倍 (P < .05)。NOX2 和 NOX4 蛋白和 mRNA 的水平在患者和对照受试者之间没有显着差异。便秘患者的 IL-1β 和 IL-6 mRNA 水平显着升高 (P < .05)，而 IL-8 mRNA 水平在两组间无差异。NADPH氧化酶和促炎细胞因子可能参与慢性功能性便秘的发病机制。与对照组相比增加了 2 倍 (P < .05)。NOX2 和 NOX4 蛋白和 mRNA 的水平在患者和对照受试者之间没有显着差异。便秘患者的 IL-1β 和 IL-6 mRNA 水平显着升高 (P < .05)，而 IL-8 mRNA 水平在两组间无差异。NADPH氧化酶和促炎细胞因子可能参与慢性功能性便秘的发病机制。