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Photoelectrochemical and visual dual-mode sensor for efficient detection of Cry1Ab protein based on the proximity hybridization driven specific desorption of multifunctional probe
Journal of Hazardous Materials ( IF 12.2 ) Pub Date : 2022-08-10 , DOI: 10.1016/j.jhazmat.2022.129759
Shuyun Meng 1 , Dong Liu 1 , Yuye Li 1 , Na Dong 1 , Shuda Liu 1 , Chang Liu 1 , Xia Li 2 , Tianyan You 1
Affiliation  

Currently, the development of sensitive and visual strategy for Cry1Ab detection, particularly using a switchable dual-mode detection system based on a single component, remains a great challenge. Here, a photoelectrochemical (PEC) and visual dual-mode sensor was designed for Cry1Ab detection based on a proximity hybridization driven multifunctional probe. In the presence of Cry1Ab, specific desorption of the antibody-DNA conjugate was achieved via sufficient proximity hybridization, leading to the selective release of the multifunctional signal probe, i.e., antibody-labeled single-stranded DNA-gold nanoparticles (Ab1-S1-AuNPs). The released Ab1-S1-AuNPs reduced the photocurrent signal and produced a colored response, thereby achieving PEC and visual dual-mode detection based on a single component. Owing to the different signal generation mechanisms, two independent signals were obtained simultaneously, which provided self-verification to improve reliability and accuracy. Taking advantage of the PEC sensitive detection and visual prediction, the dual-mode sensor achieved efficient detection of the Cry1Ab protein. The developed sensor was successfully used to determine Cry1Ab in corn, wheat, and soil samples with satisfactory results. This method offers a promising biosensing platform for the on-site detection of Cry1Ab protein.



中文翻译:

基于接近杂交驱动的多功能探针特异性解吸的光电化学和视觉双模传感器高效检测Cry1Ab蛋白

目前,开发用于 Cry1Ab 检测的灵敏和视觉策略,特别是使用基于单个组件的可切换双模式检测系统,仍然是一个巨大的挑战。在这里,基于邻近杂交驱动的多功能探针,设计了一种光电化学 (PEC) 和视觉双模传感器用于 Cry1Ab 检测。在 Cry1Ab 存在下,抗体-DNA 偶联物的特异性解吸通过充分的邻近杂交实现,导致多功能信号探针的选择性释放,即抗体标记的单链 DNA-金纳米颗粒 (Ab 1 -S1-金纳米粒子)。发布的 Ab 1-S1-AuNPs降低了光电流信号并产生了有色响应,从而实现了基于单一成分的PEC和视觉双模检测。由于不同的信号产生机制,同时获得两个独立的信号,这提供了自我验证,以提高可靠性和准确性。双模传感器利用PEC灵敏检测和视觉预测,实现了Cry1Ab蛋白的高效检测。开发的传感器成功用于测定玉米、小麦和土壤样品中的 Cry1Ab,结果令人满意。该方法为现场检测Cry1Ab蛋白提供了一个有前景的生物传感平台。

更新日期:2022-08-10
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