Currently, the development of sensitive and visual strategy for Cry1Ab detection, particularly using a switchable dual-mode detection system based on a single component, remains a great challenge. Here, a photoelectrochemical (PEC) and visual dual-mode sensor was designed for Cry1Ab detection based on a proximity hybridization driven multifunctional probe. In the presence of Cry1Ab, specific desorption of the antibody-DNA conjugate was achieved via sufficient proximity hybridization, leading to the selective release of the multifunctional signal probe, i.e., antibody-labeled single-stranded DNA-gold nanoparticles (Ab₁-S1-AuNPs). The released Ab₁-S1-AuNPs reduced the photocurrent signal and produced a colored response, thereby achieving PEC and visual dual-mode detection based on a single component. Owing to the different signal generation mechanisms, two independent signals were obtained simultaneously, which provided self-verification to improve reliability and accuracy. Taking advantage of the PEC sensitive detection and visual prediction, the dual-mode sensor achieved efficient detection of the Cry1Ab protein. The developed sensor was successfully used to determine Cry1Ab in corn, wheat, and soil samples with satisfactory results. This method offers a promising biosensing platform for the on-site detection of Cry1Ab protein.

目前，开发用于 Cry1Ab 检测的灵敏和视觉策略，特别是使用基于单个组件的可切换双模式检测系统，仍然是一个巨大的挑战。在这里，基于邻近杂交驱动的多功能探针，设计了一种光电化学 (PEC) 和视觉双模传感器用于 Cry1Ab 检测。在 Cry1Ab 存在下，抗体-DNA 偶联物的特异性解吸通过充分的邻近杂交实现，导致多功能信号探针的选择性释放，即抗体标记的单链 DNA-金纳米颗粒 (Ab ₁ -S1-金纳米粒子）。发布的 Ab ₁-S1-AuNPs降低了光电流信号并产生了有色响应，从而实现了基于单一成分的PEC和视觉双模检测。由于不同的信号产生机制，同时获得两个独立的信号，这提供了自我验证，以提高可靠性和准确性。双模传感器利用PEC灵敏检测和视觉预测，实现了Cry1Ab蛋白的高效检测。开发的传感器成功用于测定玉米、小麦和土壤样品中的 Cry1Ab，结果令人满意。该方法为现场检测Cry1Ab蛋白提供了一个有前景的生物传感平台。