The enzyme β-galactosidase (EC 3.2.1.2.3) obtained from yeast is produced intracellularly. Cell extraction consists of the treatment of a cell suspension with chemical agents, thus obtaining the enzyme with biological activity. After obtaining the extracted enzyme, it is concentrated by ultrafiltration and dried. Lyophilization can be used in this step. The objective of this work was to study the production and extraction of β-galactosidase from Saccharomyces fragilis IZ 275 using lactose from cheese whey.

中文翻译：

---

从生长在奶酪乳清中的脆弱酵母 IZ 275 中提取 β-半乳糖苷酶

从酵母中获得的酶 β-半乳糖苷酶 (EC 3.2.1.2.3) 在细胞内产生。细胞提取包括用化学试剂处理细胞悬浮液，从而获得具有生物活性的酶。得到提取的酶后，超滤浓缩，干燥。在此步骤中可以使用冻干。这项工作的目的是研究使用奶酪乳清中的乳糖从脆弱酵母IZ 275 中生产和提取 β-半乳糖苷酶。