A low-cost and straightforward hybrid NOA (Norland optical adhesive) 81-glass microchip electrophoresis device was designed and developed for protein separation using indirect fluorescence detection. This new microchip was first characterized in terms of surface charge density via electroosmotic mobility measurement and stability over time. A systematic determination of the electroosmotic mobility (μ_eo) over a wide pH range (2–10) and at various ionic strengths (20–50 mM) was developed for the first time via the neutral marker approach in an original simple frontal methodology. The evolution of μ_eo was proved consistent with the silanol and thiol functions arising from the glass and the NOA materials, respectively. The repeatability and reproducibility of the measurements on different microchips (RSD < 14%) and within 15 days (less than 5% decrease) were successfully demonstrated. The microchip was then applied for the efficient electrophoretic separation of proteins in a zonal mode coupled with indirect fluorescence detection, which is, to our knowledge, the first proof of concept of capillary zone electrophoresis in this hybrid microsystem.

中文翻译：

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混合 NOA 81 玻璃微芯片的电动特性：在具有间接荧光检测的蛋白质微芯片电泳中的应用

设计和开发了一种低成本且直接的混合 NOA（Norland 光学粘合剂）81 玻璃微芯片电泳装置，用于使用间接荧光检测进行蛋白质分离。这种新的微芯片首先通过电渗迁移率测量的表面电荷密度和随时间的稳定性进行了表征。通过原始简单的正面方法中的中性标记方法，首次开发了在广泛的 pH 范围 (2-10) 和各种离子强度 (20-50 mM)下的电渗迁移率 ( μ _eo )的系统测定。μeo_的进化被证明分别与玻璃和 NOA 材料产生的硅醇和硫醇功能一致。成功证明了在不同微芯片上（RSD < 14%）和 15 天内（减少小于 5%）测量的重复性和再现性。然后将该微芯片应用于以带状模式结合间接荧光检测对蛋白质进行有效电泳分离，据我们所知，这是该混合微系统中毛细管带电泳概念的第一个证明。