Bioaccessibility of Microplastic-Associated Antibiotics in Freshwater Organisms: Highlighting the Impacts of Biofilm Colonization via an In Vitro Protocol,Environmental Science & Technology

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Bioaccessibility of Microplastic-Associated Antibiotics in Freshwater Organisms: Highlighting the Impacts of Biofilm Colonization via an In Vitro Protocol
Environmental Science & Technology ( IF 10.8 ) Pub Date : 2022-08-11 , DOI: 10.1021/acs.est.2c02782
Peng Liu _{1,

2} , Jiamin Dai ₁ , Chunyao Bie ₁ , Huang Li ₁ , Zixuan Zhang ₁ , Xuetao Guo _{1,

2} , Lingyan Zhu _{1,

2}

Affiliation

Microplastics in the environment can be colonized by microbes capable of forming biofilms, which may act as reactive coatings to affect the bioaccessibility of pollutants in organisms. This study investigated the dynamic evolution of biofilm colonization on microplastics and its impacts and mechanisms on the bioaccessibility of microplastic-associated sulfamethazine (SMT) via microcosm incubation in surface water and sediment. After 60 days of incubation, the microbial communities formed in microplastics were distinct and more diverse than those untethered in surroundings, and photoaging treatment decreased the affinity of biofilms on microplastics due to decreased hydrophobicity. Biofilm formation further enhanced the desorption and bioaccessibility of microplastic-sorbed SMT in organisms. In vitro experiments indicated that the critical effects were mainly related to the stronger interaction of gastrointestinal components (i.e., pepsin, bovine serum albumin (BSA), and NaT) with biofilm components (e.g., extracellular polymer substances) than with the pure surface of microplastics, which competed for binding sites in microplastics for SMT more significantly. Photoaging decreased the enhancing effects of biofilms due to their lower accumulation in aged microplastics. This study is the first attempt to reveal the role of biofilms in the bioaccessibility of microplastics with associated antibiotics and provide insights into the combined risk of microplastics in the environment.

中文翻译：

微塑料相关抗生素在淡水生物中的生物可及性：通过体外方案突出生物膜定植的影响

环境中的微塑料可以被能够形成生物膜的微生物定殖，这些微生物可以作为反应性涂层来影响生物体中污染物的生物可及性。本研究通过在地表水和沉积物中的微观孵化研究了生物膜定植在微塑料上的动态演变及其对微塑料相关磺胺二甲嘧啶 (SMT) 生物可及性的影响和机制。经过 60 天的孵化，在微塑料中形成的微生物群落比在周围环境中形成的微生物群落更加明显和多样化，并且由于疏水性降低，光老化处理降低了生物膜对微塑料的亲和力。生物膜的形成进一步增强了微塑料吸附的 SMT 在生物体中的解吸和生物可及性。体外实验表明，与微塑料纯表面相比，胃肠道成分（即胃蛋白酶、牛血清白蛋白（BSA）和NaT）与生物膜成分（例如细胞外聚合物物质）的相互作用更强。，它更显着地竞争 SMT 微塑料中的结合位点。光老化降低了生物膜的增强效果，因为它们在老化的微塑料中积累较少。这项研究首次尝试揭示生物膜在微塑料与相关抗生素的生物可及性中的作用，并提供对环境中微塑料综合风险的见解。

更新日期：2022-08-11

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