Traditional strategies for coupling of proteins with DNA involve the additional modifications on protein or DNA to construct protein-DNA conjugates, resulting in complex or time-consuming coupling process. This study presented a biomimetic synthesis strategy to elaborately synthesize a new type of biomolecule-inorganic hybrid nanosheets. Horseradish peroxidase (HRP) and DNA aptamer can be easily combined with CaHPO₄ via coprecipitation simultaneously to form all-inclusive HRP-aptamer-CaHPO₄ hybrid (HAC) nanosheets integrating bifunction of biorecognition and signal amplification, which was proceeded in the green environment at room temperature and required no additional modifications on CaHPO₄, protein and DNA. Therefore, it avoided tedious linking and purification procedures. The HAC nanosheets were then employed as the signal labels and showed excellent performance for detecting thrombin. This bioinspired approach provides great possibilities to facile and efficient immobilization of protein, DNA or even other types of biomolecules (e.g., RNA and peptide) on inorganic nanomaterials and endows great potential in the preparation of a variety of multifunctional biomolecule-CaHPO₄ two-dimensional (2D) nanobiohybrids for various applications extending from biosensing to energy, biomedicine, environmental science and catalysis.

蛋白质与 DNA 偶联的传统策略涉及对蛋白质或 DNA 进行额外修饰以构建蛋白质-DNA 偶联物，从而导致复杂或耗时的偶联过程。本研究提出了一种仿生合成策略，精心合成了一种新型的生物分子-无机杂化纳米片。辣根过氧化物酶（HRP）和DNA适体可以很容易地通过同时共沉淀与CaHPO ₄结合，形成集生物识别和信号放大双重功能的包罗万象的HRP-适体-CaHPO ₄杂化（HAC）纳米片。室温，无需对 CaHPO _{4进行额外修改}、蛋白质和DNA。因此，它避免了繁琐的连接和纯化程序。然后将 HAC 纳米片用作信号标记，并在检测凝血酶方面表现出优异的性能。这种仿生方法为将蛋白质、DNA甚至其他类型的生物分子（如RNA和肽）简单有效地固定在无机纳米材料上提供了巨大的可能性，并赋予了制备多种多功能生物分子-CaHPO ₄二维的巨大潜力(2D) 用于从生物传感到能源、生物医学、环境科学和催化的各种应用的纳米生物杂化物。