During 2021, we collected blood and serum samples from 135 persons exposed to tick bites in southern Italy. We serologically and molecularly screened for zoonotic tickborne pathogens and molecularly screened for Candidatus Midichloria mitochondrii. Overall, 62 (45.9%) persons tested positive for tickborne pathogens. Coxiella burnetii was detected most frequently (27.4%), along with Rickettsia spp. (21.5%) and Borrelia spp. (10.4%). We detected Candidatus M. mitochondrii DNA in 46 (34.1%) participants who had statistically significant associations to tickborne pathogens (p<0.0001). Phylogenetic analysis of Candidatus M. mitochondrii sequences revealed 5 clades and 8 human sequence types that correlated with vertebrates, Ixodes spp. ticks, and countries in Europe. These data demonstrated a high circulation of tickborne pathogens and Candidatus M. mitochondrii DNA in persons participating in outdoor activities in southern Italy. Our study shows how coordinated surveillance among patients, clinicians, and veterinarians could inform a One Health approach for monitoring and controlling the circulation of tickborne pathogens.

2021 年，我们收集了意大利南部 135 名接触过蜱叮咬的人的血液和血清样本。我们对人畜共患蜱传病原体进行血清学和分子筛检，并对线粒体念珠菌进行分子筛检。总体而言，62 人（45.9%）的蜱传病原体检测呈阳性。Coxiella burnetii最常见（27.4%），以及立克次体。(21.5%) 和疏螺旋体属。(10.4%)。我们在 46 名 (34.1%) 参与者中检测到Candidatus M. mitochondrii DNA，这些参与者与蜱传病原体具有统计学显着相关性 (p<0.0001)。候选者的系统发育分析M. mitochondrii 序列揭示了与脊椎动物Ixodes spp相关的 5 个进化枝和 8 个人类序列类型。蜱虫和欧洲国家。这些数据表明，在意大利南部参加户外活动的人中，蜱传病原体和Candidatus M. mitochondrii DNA 的高循环。我们的研究表明，患者、临床医生和兽医之间的协调监测如何为监测和控制蜱传病原体循环的 One Health 方法提供信息。