Small RNAs regulate spermatogenesis across species ranging from Caenorhabditis elegans to humans. In C. elegans , two Argonaute proteins, ALG-3 and ALG-4, and their associated alg-3/4 26G-small RNAs are essential for spermiogenesis at 25°C. The alg-3/4 26G-small RNAs are antisense to their target mRNAs and produced by the RNA-dependent RNA polymerase, RRF-3. However, it remains unclear how the RNA templates for RRF-3 are generated and which cellular processes are affected by alg-3/4 26G-small RNAs. Here, we demonstrate a previously unidentified zc3h12a-like ribonuclease protein, NYN-3, in alg-3/4 26G-small RNAs biogenesis. NYN-3 is not only required for proper abundance of alg-3/4 26G-small RNAs but also crosslinked to their targeted mRNAs before RRF-3 from ePAR-CLIP-seq. Bioinformatics analysis was then used to parse the 26G-small RNA-targeted genes into functional subclasses. Collectively, these findings implicate NYN-3 as an initiator of alg-3/4 26G-small RNA generation.

中文翻译：

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秀丽隐杆线虫精子发生小 RNA 的生物发生由 zc3h12a 样核糖核酸酶启动

小 RNA 调节跨物种的精子发生，范围从秀丽隐杆线虫对人类。在秀丽隐杆线虫，两种 Argonaute 蛋白，ALG-3 和 ALG-4，以及它们相关的alg-3/426G 小 RNA 在 25°C 下对精子发生至关重要。这alg-3/426G 小 RNA 与其靶 mRNA 是反义的，由 RNA 依赖性 RNA 聚合酶 RRF-3 产生。然而，目前尚不清楚 RRF-3 的 RNA 模板是如何产生的，以及哪些细胞过程受其影响。alg-3/426G-小 RNA。在这里，我们展示了一种以前未知的 zc3h12a 样核糖核酸酶蛋白 NYN-3alg-3/426G-小 RNA 的生物发生。NYN-3 不仅需要适当的丰度alg-3/426G 小 RNA，但也在来自 ePAR-CLIP-seq 的 RRF-3 之前与它们的目标 mRNA 交联。然后使用生物信息学分析将 26G 小 RNA 靶向基因解析为功能亚类。总的来说，这些发现暗示 NYN-3 是alg-3/426G-小 RNA 生成。