Bucket chlorination, where chlorine is dosed directly into water collection containers, is a point-of-source water treatment intervention commonly implemented in cholera outbreaks. There is little previous data on chlorine efficacy against Vibrio cholerae in different waters and appropriate dosage regimes. We evaluated V. cholerae reduction and free chlorine residual (FCR) in waters with four turbidities (1/5/10/50 NTU), two total organic carbon (TOC) concentrations (0.4, 1 mg/L), and two dosing schemes (fixed-dose of 2 or 4 mg/L, variable-dose based on jar testing) treated with three chlorine types (HTH, NaOCl, NaDCC). We found that chlorine was efficacious at reducing V. cholerae by ≥2.75 to ≥3.63 log reduction value (LRV); variably dosed reactors were dosed higher, met ≥0.5 mg/L FCR at 30 min, and had higher LRVs (p=0.024) than fixed doses; and low TOC reactors had more samples ≥0.2 mg/L FRC at 4 h (p=0.007). Our results are conservative, as internationally recommended additives to create test water increased chlorine demand, highlighting the challenge of replicating field conditions in laboratory testing. Overall, we found that chlorine can efficaciously reduce V. cholerae; we recommend further research on appropriate chlorine demand for test waters; and we recommend establishing appropriate chlorine doses based on source water and taste/odor acceptability in bucket chlorination programs.

桶式氯化法，即直接将氯加入集水容器中，是霍乱爆发中通常实施的一种源头水处理干预措施。在不同的水域和适当的剂量方案中，关于氯对霍乱弧菌的功效的先前数据很少。我们评估了四种浊度 (1/5/10/50 NTU)、两种总有机碳 (TOC) 浓度 (0.4, 1 mg/L) 和两种给药方案的水中的霍乱弧菌减少和游离氯残留 (FCR) （2 或 4 mg/L 的固定剂量，基于罐测试的可变剂量）用三种氯类型（HTH、NaOCl、NaDCC）处理。我们发现氯能有效减少霍乱弧菌≥2.75 至 ≥3.63 对数减少值 (LRV)；与固定剂量相比，可变剂量反应器的剂量更高，在 30 分钟时达到≥0.5 mg/L FCR，并且具有更高的 LRV（p = 0.024）；和低 TOC 反应器在 4 小时有更多样品≥0.2 mg/L FRC ( p =0.007)。我们的结果是保守的，因为国际推荐的用于产生测试水的添加剂增加了氯需求，突出了在实验室测试中复制现场条件的挑战。总的来说，我们发现氯可以有效减少霍乱弧菌；我们建议进一步研究测试水的适当氯需求；我们建议根据水桶氯化程序中的水源和味道/气味可接受性确定适当的氯剂量。