Root Hair Apex is the Key Site for Symplastic Delivery of Graphene into Plants,Environmental Science & Technology

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Root Hair Apex is the Key Site for Symplastic Delivery of Graphene into Plants
Environmental Science & Technology ( IF 10.8 ) Pub Date : 2022-08-10 , DOI: 10.1021/acs.est.2c01926
Shipeng Dong ₁ , Xueping Jing ₁ , Sijie Lin ₂ , Kun Lu ₁ , Wenfei Li ₃ , Jiajun Lu ₃ , Muzi Li ₁ , Shixiang Gao ₁ , Shan Lu ₄ , Dongmei Zhou ₁ , Chunying Chen ₅ , Baoshan Xing ₆ , Liang Mao ₁

Affiliation

Uptake kinetics and delivery mechanisms of nanoparticles (NPs) in crop plants need to be urgently understood for the application of nanotechnology in agriculture as delivery systems for eco-friendly nanoagrochemicals. Here, we investigated the uptake kinetics, translocation pathway, and key internalization process of graphene in wheat (Triticum aestivumL.) by applying three specific hydroponic cultivation methods (submerging, hanging, and split-root). Quantification results on the uptake of carbon-14 radiolabeled graphene in each tissue indicated that graphene could enter the root of wheat and further translocate to the shoot with a low delivery rate (<2%). Transmission electron microscopy (TEM) images showed that internalized graphene was transported to adjacent cells through the plasmodesmata, clearly indicating the symplastic pathway of graphene translocation. The key site for the introduction of graphene into root cells for translocation through the symplastic pathway is evidenced to be the apex of growing root hair, where the newly constructed primary cell wall is much thinner. The confirmation of uptake kinetics and delivery mechanisms is useful for the development of nanotechnology in sustainable agriculture, especially for graphene serving as the delivery vector for pesticides, genes, and sensors.

中文翻译：

根毛尖是石墨烯共塑性传递到植物中的关键位点

纳米颗粒（NPs）在农作物中的吸收动力学和递送机制需要迫切了解，以便将纳米技术作为生态友好型纳米农用化学品的递送系统在农业中的应用。在这里，我们研究了石墨烯在小麦（Triticum aestivum L.) 通过应用三种特定的水耕栽培方法（浸没、悬挂和分根）。对每个组织中碳 14 放射性标记的石墨烯吸收的量化结果表明，石墨烯可以进入小麦的根部并进一步转移到地上部，且递送率较低（<2%）。透射电子显微镜 (TEM) 图像显示内化的石墨烯通过胞间连丝转运到相邻细胞，清楚地表明石墨烯易位的共生途径。将石墨烯引入根细胞以通过共生途径易位的关键位点被证明是正在生长的根毛的顶端，其中新构建的初级细胞壁要薄得多。

更新日期：2022-08-10

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