Using several types of Pt-porphyrin based oxygen sensing materials and conditions, we evaluated comparatively four different detection platforms that perform phosphorescence lifetime (PLT) measurements in the microsecond time domain. The time-resolved fluorescence reader Victor 2, which utilises Rapid Lifetime Determination method and Xe-flash lamp excitation, produced stable but inaccurate readings at PLT values < 15 µs, affected by the flash-lamp afterglow and sensor heterogeneity in oxygenated conditions. The luminescence spectrometer Cary Eclipse, which utilises multi-point phosphorescence decay method, was able to partly correct these interfering factors and produce more accurate PLT values from mono- and bi-exponential fits, however short PLT values were still affected by Xe-flash lamp afterglow. The wide-field Tpx3Cam cam-era-based macroscope and the confocal laser-scanning FLIM/PLIM microscope, which use short-pulse excitation (ns LED or ps diode laser pulses, respectively) and TCSPC method, revealed the presence of short-lived components (1.5–6.0 µs) in sample decays, which have to be considered in PLT calculations. The imaging platforms produced more accurate and in-formative PLT data, but they operate with large volumes of raw and processed data. Overall, for each PLT platform a number of potential sources of error and measurement artefacts have been revealed, which can be minimised by careful optimisation of settings and validation with alternative platforms and sensor materials.

使用几种类型的基于铂卟啉的氧传感材料和条件，我们评估了四种不同的检测平台，它们在微秒时域内进行磷光寿命 (PLT) 测量。时间分辨荧光读数器 Victor 2 采用快速寿命测定方法和氙闪光灯激发，在 PLT 值 < 15 µs 时产生稳定但不准确的读数，受闪光灯余辉和传感器在含氧条件下的异质性的影响。采用多点磷光衰减法的发光光谱仪 Cary Eclipse 能够部分校正这些干扰因素，并通过单指数和双指数拟合产生更准确的 PLT 值，但短 PLT 值仍然受到 Xe 闪光灯的影响余辉。使用短脉冲激发（分别为 ns LED 或 ps 二极管激光脉冲）和 TCSPC 方法的宽视场 Tpx3Cam 基于相机的宏观显微镜和共焦激光扫描 FLIM/PLIM 显微镜揭示了短寿命的存在样本衰减中的分量 (1.5–6.0 µs)，在 PLT 计算中必须考虑。成像平台产生了更准确和信息量更大的 PLT 数据，但它们使用大量原始数据和处理过的数据。总体而言，对于每个 PLT 平台，已经揭示了许多潜在的误差来源和测量伪影，可以通过仔细优化设置和使用替代平台和传感器材料进行验证来将其最小化。揭示了样品衰减中存在短寿命成分（1.5-6.0 µs），在 PLT 计算中必须考虑这些成分。成像平台产生了更准确和信息量更大的 PLT 数据，但它们使用大量原始数据和处理过的数据。总体而言，对于每个 PLT 平台，已经揭示了许多潜在的误差来源和测量伪影，可以通过仔细优化设置和使用替代平台和传感器材料进行验证来将其最小化。揭示了样品衰减中存在短寿命成分（1.5-6.0 µs），在 PLT 计算中必须考虑这些成分。成像平台产生了更准确和信息量更大的 PLT 数据，但它们使用大量原始数据和处理过的数据。总体而言，对于每个 PLT 平台，已经揭示了许多潜在的误差来源和测量伪影，可以通过仔细优化设置和使用替代平台和传感器材料进行验证来将其最小化。