Chronic inflammatory wounds pose therapeutic challenges in the biomedical field. Polymeric nanofibrous matrices provide extracellular-matrix-like structures to facilitate wound healing; however, wound infection and the subsequent accumulation of reactive oxygen species (ROS) delay healing. Therefore, we herein developed electrospun nanofibers (NFs), composed of chitosan-stabilized Prussian blue (PBChi) nanoparticles (NPs) and poly(vinyl alcohol) (PVA), with ROS scavenging activity to impart antioxidant and wound healing properties. The PBChi NPs were prepared using chitosan with different molecular weights, and their weight ratio with respect to PVA was optimized to yield PBChi-NP-coated PVA NFs with well-defined NF structures. In situ and in vitro antioxidant activity assays showed that the PBChi/PVA NFs could effectively remove ROS. Particularly, PBChi/PVA NFs with a lower chitosan molecular weight exhibited greater antioxidant activity. The hydroxyl radical scavenging activity of PBChi10k/PVA NFs was 60.4 %, approximately two-fold higher than that of PBChi100k/PVA NFs. Further, at the concentration of 10 μg/mL, they could significantly lower the in vitro ROS level by up to 50.7 %. The NFs caused no significant reduction in cell viability, owing to the excellent biocompatibility of PVA with PBChi NPs. Treatment using PBChi/PVA NFs led to faster cell proliferation in in vitro scratch wounds, reducing their size from 202 to 162 μm. The PBChi/PVA NFs possess notable antioxidant and cell proliferation properties as ROS-scavenging wound dressings.

慢性炎症性伤口在生物医学领域提出了治疗挑战。聚合物纳米纤维基质提供细胞外基质样结构以促进伤口愈合；然而，伤口感染和随后的活性氧 (ROS) 积累会延迟愈合。因此，我们在此开发了由壳聚糖稳定的普鲁士蓝 (PBChi) 纳米粒子 (NPs) 和聚乙烯醇 (PVA) 组成的静电纺丝纳米纤维 (NFs)，具有清除活性氧的活性以赋予抗氧化和伤口愈合特性。使用不同分子量的壳聚糖制备 PBChi NPs，并优化它们与 PVA 的重量比以产生具有明确 NF 结构的 PBChi-NP 包覆的 PVA NFs。原位和体外抗氧化活性测定表明，PBChi/PVA NFs 可以有效去除 ROS。特别是，具有较低壳聚糖分子量的 PBChi/PVA NFs 表现出更大的抗氧化活性。PBChi10k/PVA NFs 的羟基自由基清除活性为60.4%，大约是PBChi100k/PVA NFs 的两倍。此外，在 10 μg/mL 的浓度下，它们可以显着降低体外ROS 水平高达 50.7%。由于 PVA 与 PBChi NPs 具有优异的生物相容性，NFs 不会显着降低细胞活力。使用 PBChi/PVA NFs 治疗导致体外细胞增殖更快划伤，将其尺寸从 202 微米减小到 162 微米。PBChi/PVA NFs 作为 ROS 清除伤口敷料具有显着的抗氧化和细胞增殖特性。