Previous studies have shown that AQP9 plays an important role in energy metabolism in nonalcoholic fatty liver disease (NAFLD). Recently, metabolomic analyses were used to determine the slight changes in metabolic profiles and helped to understand the disease progression, therapeutic intervention of NAFLD. A mouse model of NAFLD was established with a high-fat diet (HFD), and Aqp9 knockout mice were constructed. Untargeted metabolomics techniques were used to evaluate the potential mechanism of the effect of AQP9 in NAFLD. The results indicated that AQP9 plays a regulatory role in the occurrence of NAFLD. Moreover, a total of 220 candidate biomarkers were screened and identified. Cluster analysis and enrichment analysis of differential metabolites indicated that fatty acid biosynthesis was mainly disturbed when compared against the control group, which was mitigated by knockout of Aqp9. These results show that untargeted metabolomics help to understand the effects of AQP9 in NAFLD.

以往的研究表明，AQP9 在非酒精性脂肪性肝病 (NAFLD) 的能量代谢中起重要作用。最近，代谢组学分析被用于确定代谢谱的微小变化，并有助于了解 NAFLD 的疾病进展和治疗干预。采用高脂饮食（HFD）和Aqp9建立 NAFLD 小鼠模型构建了敲除小鼠。非靶向代谢组学技术用于评估 AQP9 在 NAFLD 中作用的潜在机制。结果表明AQP9在NAFLD的发生中起调节作用。此外，共筛选和鉴定了 220 个候选生物标志物。差异代谢物的聚类分析和富集分析表明，与对照组相比，脂肪酸生物合成主要受到干扰，通过敲除Aqp9可以减轻这种干扰。这些结果表明，非靶向代谢组学有助于了解 AQP9 在 NAFLD 中的作用。