In this project, different photosensitizers were prepared using different ratios of nickel, manganese, and iron. Then, multiple analysis were performed to evaluate their efficiency, and the most suitable one was used to be coated by hyaluronic acid to improve the nano-platform's biocompatibility and target ability. Moreover, another chemical targeting agent (riboflavin) was used to further improve the target ability of the prepared nano-platform. Different spectroscopies and thermal analysis were used to determine the physical and chemical characteristics of the prepared nano-platform. Also, in order to determine the biocompatibility of the nano-platform, in vitro and in vivo tests such as blood hemolysis, blood aggregation and lethal dose were performed. Then, an anti-cancer agent (curcumin) was loaded on the selected nano-platform to makes us able utilizing the synergistic effect of chemotherapy and photodynamic therapy simultaneously. Finally, the cell cytotoxicity results showed that the prepared nano-platform had a great anti-cancer potential which can make it a great candidate as a dual photo and chemo therapy agent for treatment of breast cancers.

在这个项目中，使用不同比例的镍、锰和铁制备了不同的光敏剂。然后，进行了多次分析以评估它们的效率，最合适的一种被用于透明质酸包被，以提高纳米平台的生物相容性和靶向能力。此外，还使用了另一种化学靶向剂（核黄素）来进一步提高制备的纳米平台的靶向能力。使用不同的光谱和热分析来确定制备的纳米平台的物理和化学特性。此外，为了确定纳米平台在体外和体内的生物相容性进行了血液溶血、血液聚集和致死剂量等测试。然后，将抗癌剂（姜黄素）加载到选定的纳米平台上，使我们能够同时利用化学疗法和光动力疗法的协同作用。最后，细胞毒性结果表明，制备的纳米平台具有很大的抗癌潜力，可以作为治疗乳腺癌的光化学双重治疗剂的理想候选者。