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In vitro simulated digestion affecting physicochemical characteristics and bioactivities of polysaccharides from barley (Hordeum vulgare L.) grasses at different growth stages
International Journal of Biological Macromolecules ( IF 7.7 ) Pub Date : 2022-08-10 , DOI: 10.1016/j.ijbiomac.2022.08.043
Jing-Kun Yan 1 , Ting-Ting Chen 2 , Lin Wang 2 , Zi-Wei Wang 2 , Can Li 2 , Wen-Yan Chen 2 , Chenghong Liu 3 , Lin Li 4
Affiliation  

In this study, three polysaccharides (BGPs: BGPs-Z21, BGPs-Z23, and BGPs-Z31) were successively extracted from barley (Hordeum vulgare L.) grasses (BG) at different growth stages, including seedling (Z21), tillering (Z23), and stem elongation (Z31). The effects of in vitro simulated saliva–gastrointestinal digestion on the physicochemical characteristics and biological activities of BGPs were investigated and compared. Results showed that the simulated saliva–gastrointestinal digestion had considerable influences on reducing sugar content, chemical components, monosaccharide constituents, and molecular weights of BGPs but hardly affected their preliminarily structural characteristics. Moreover, the antioxidant activities of BGPs were weakened after the simulated saliva–gastrointestinal digestion, but their bile acid-binding capacities were remarkably enhanced. The digested BGPs-Z31 by gastric juice possessed better antioxidant benefit, and bile acid-binding capacity (80.33 %) than other digested products. Overall, these results indicated that BGPs obtained from BG are valuable for functional foods as promising bioactive ingredients.



中文翻译:

体外模拟消化对不同生长阶段大麦(Hordeum vulgare L.)草多糖理化特性和生物活性的影响

本研究从大麦(Hordeum vulgare L.)草(BG)的不同生长阶段,包括幼苗(Z21)、分蘖( Z23) 和茎伸长率 (Z31)。体外的影响研究比较了模拟唾液-胃肠消化对BGPs的理化特性和生物学活性的影响。结果表明,模拟唾液-胃肠消化对BGPs的还原糖含量、化学成分、单糖成分和分子量有相当大的影响,但对其初步结构特征几乎没有影响。此外,模拟唾液-胃肠道消化后,BGPs的抗氧化活性减弱,但胆汁酸结合能力显着增强。胃液消化的BGPs-Z31比其他消化产物具有更好的抗氧化作用和胆汁酸结合能力(80.33%)。全面的,

更新日期:2022-08-13
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