Abstract

Landfill leachate, due to its recalcitrant nature and toxicity, poses a serious environmental threat, which requires the implementation of effective treatment processes. In this work, a full-scale treatment system consisting of two Sequencing Batch Reactors (SBRs) was used for the processing of landfill leachate of intermediate to mature age (BOD/COD ratio of 0.16). Biosystem operation resulted in BOD₅, COD and TKN removal efficiencies of 81%, 39% and 76%, respectively, whereas the low residual NO₃^--N concentration in the effluent (4.01 ± 0.10 mg/L) was indicative of the efficient denitrification process. Assessment of hydrolytic potential of activated sludge revealed high endocellular and extracellular lipase activities, which reached values up to 206 and 141 U/g protein respectively, possibly as the consequence of plastics degradation during maturation process. Implementation of Illumina sequencing indicated the predominance of Alphaproteobacteria, accompanied by members of Bacteroidetes, Betaproteobacteria and Chloroflexi. Paracoccus was the predominant genus identified, followed by representatives of the genera Bellilinea, Flavobacterium, Thauera and Truepera. Nitrosomonas was the major ammonia-oxidizing bacterium (AOB), while nitrite oxidation was mainly achieved by the uncultured nitrite-oxidizing bacterium (NOB) Candidatus Nitrotoga.

摘要

垃圾渗滤液由于其顽固性和毒性，对环境构成严重威胁，需要实施有效的处理工艺。在这项工作中，一个由两个序批式反应器 (SBR) 组成的全面处理系统用于处理中等至成熟年龄的垃圾渗滤液（BOD/COD 比为 0.16）。生物系统运行导致 BOD ₅、COD 和 TKN 去除效率分别为 81%、39% 和 76%，而低残留 NO ₃ ^-流出物中的 -N 浓度 (4.01 ± 0.10 mg/L) 表明了有效的反硝化过程。对活性污泥水解潜力的评估显示细胞内和细胞外脂肪酶活性较高，分别达到 206 和 141 U/g 蛋白质，这可能是成熟过程中塑料降解的结果。Illumina测序的实施表明Alphaproteobacteria占优势，同时有Bacteroidetes、Betaproteobacteria和Chloroflexi的成员。Paracoccus是鉴定的主要属，其次是Bellilinea属、黄杆菌属、Thauera和Truepera。亚硝化单胞菌是主要的氨氧化细菌（AOB），而亚硝酸盐氧化主要由未培养的亚硝酸盐氧化细菌（NOB）Candidatus Nitrotoga 实现。