Entomopathogenic fungi (EPFs) have more important role in biological control of the important insect pests. In the present study 15 EPF isolates, selected from the EPF culture collection, were identified using molecular methods and their effectiveness against adults of Apple Blossom Beetle (ABB) [Tropinota (= Epicometis) hirta (Poda, 1761) (Coleoptera: Cetoniidae)] was investigated. To determine virulence of isolates against ABB adults, primarily a single-concentration trial was conducted at 1 × 107 conidia ml−1. Furthermore, concentration-mortality trials were conducted with some isolates that were determined to have a high effect, employing concentrations of 1 × 106, 1 × 108, and 1 × 109 conidia ml−1. For isolates tested in concentration-mortality trials, LT30, LT50 and LT90 values at a concentration of 1 × 108 conidia ml−1 were calculated using probit analysis. Molecular diagnostic tests revealed that the 13 isolates were Beauveria bassiana, 1 isolate was Clonostachys rosea, and 1 isolate was Purpureocillium lilacinum. According to the single- concentration trial data, some isolates with high efficacy were selected (GN16, GN22-1, GN29-1, HP3-1 and HP33-1) and concentration-mortality trials were established with these isolates using 1 × 106, 1 × 108 and 1 × 109 conidia ml−1 concentrations. According to the results of the concentration-mortality trials, all isolates used in 1 × 108 and 1 × 109 conidia ml−1 concentrations started to show a significant effect from the 11th day, and at the end of the 17th day, the effect in all concentrations was over 90% (except 1 × 106 conidia ml−1 concentration of GN22-1). According to the data obtained, all isolates used in concentration-mortality trials were effective against ABB adults. With these isolates, more detailed studies should be conducted under orchard conditions.

中文翻译：

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一些原生昆虫病原真菌物种的鉴定及其对苹果花甲虫、Tropinota (Epicometis) hirta (Poda, 1761) (鞘翅目: Cetoniidae) 成虫的致病性

昆虫病原真菌（EPFs）在重要害虫的生物防治中发挥着更为重要的作用。在本研究中，使用分子方法鉴定了从 EPF 培养物收集中选择的 15 种 EPF 分离物及其对苹果花甲虫 (ABB) [Tropinota (= Epicometis) hirta (Poda, 1761) (鞘翅目：Cetoniidae)] 成虫的有效性。进行了调查。为了确定分离株对 ABB 成虫的毒力，主要在 1 × 107 分生孢子 ml-1 下进行了单浓度试验。此外，使用浓度为 1 × 106、1 × 108 和 1 × 109 分生孢子 ml-1 的一些分离株进行了浓度死亡率试验，这些分离株被确定为具有高影响。对于在浓度死亡率试验中测试的分离株，LT30，使用概率分析计算浓度为 1 × 108 分生孢子 ml-1 的 LT50 和 LT90 值。分子诊断检测结果显示，13株分离株为白僵菌，1株株为玫瑰花序，1株株为Purpureocillium lilacinum。根据单浓度试验数据，选择了一些高效的分离株（GN16、GN22-1、GN29-1、HP3-1和HP33-1），并使用1×106对这些分离株建立浓度-死亡率试验， 1 × 108 和 1 × 109 分生孢子 ml-1 浓度。根据浓度-死亡率试验的结果，在 1 × 108 和 1 × 109 分生孢子 ml-1 浓度中使用的所有分离株从第 11 天开始显示出显着效果，到第 17 天结束时，所有浓度的效果均超过 90%（GN22-1 的 1 × 106 分生孢子 ml-1 浓度除外）。根据获得的数据，用于浓度死亡率试验的所有分离株均对 ABB 成人有效。对于这些分离株，应在果园条件下进行更详细的研究。