All-Optical Modulation of Single Defects in Nanodiamonds: Revealing Rotational and Translational Motions in Cell Traction Force Fields,Nano Letters

当前位置： X-MOL 学术 › Nano Lett. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

All-Optical Modulation of Single Defects in Nanodiamonds: Revealing Rotational and Translational Motions in Cell Traction Force Fields
Nano Letters ( IF 9.6 ) Pub Date : 2022-08-10 , DOI: 10.1021/acs.nanolett.2c02232
Lingzhi Wang ₁ , Yong Hou ₁ , Tongtong Zhang ₁ , Xi Wei ₂ , Yan Zhou ₃ , Dangyuan Lei ₄ , Qiang Wei ₅ , Yuan Lin ₂ , Zhiqin Chu _{1,

6}

Affiliation

Measuring the mechanical interplay between cells and their surrounding microenvironment is vital in cell biology and disease diagnosis. Most current methods can only capture the translational motion of fiduciary markers in the deformed matrix, but their rotational motions are normally ignored. Here, by utilizing single nitrogen-vacancy (NV) centers in nanodiamonds (NDs) as fluorescent markers, we propose a linear polarization modulation (LPM) method to monitor in-plane rotational and translational motions of the substrate caused by cell traction forces. Specifically, precise orientation measurement and localization with background suppression were achieved via optical polarization selective excitation of single NV centers with precisions of ∼0.5°/7.5 s and 2 nm/min, respectively. Additionally, we successfully applied this method to monitor the multidimensional movements of NDs attached to the vicinity of cell focal adhesions. The experimental results agreed well with our theoretical calculations, demonstrating the practicability of the NV-based LPM method in studying mechanobiology and cell-material interactions.

中文翻译：

纳米金刚石中单个缺陷的全光调制：揭示细胞牵引力场中的旋转和平移运动

测量细胞与其周围微环境之间的机械相互作用对于细胞生物学和疾病诊断至关重要。目前的大多数方法只能捕获变形矩阵中基准标记的平移运动，但通常会忽略它们的旋转运动。在这里，通过利用纳米金刚石 (ND) 中的单个氮空位 (NV) 中心作为荧光标记，我们提出了一种线性偏振调制 (LPM) 方法来监测由细胞牵引力引起的基板的面内旋转和平移运动。具体而言，通过单个 NV 中心的光学偏振选择性激发实现了具有背景抑制的精确定向测量和定位，精度分别为 ~0.5°/7.5 s 和 2 nm/min。此外，我们成功地应用这种方法来监测附着在细胞粘着斑附近的 ND 的多维运动。实验结果与我们的理论计算非常吻合，证明了基于 NV 的 LPM 方法在研究力学生物学和细胞-材料相互作用中的实用性。

更新日期：2022-08-10

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11