Wheat stripe rust and powdery mildew are important worldwide diseases of wheat (Triticum aestivum). The wheat cultivar Xingmin318 (XM318) is resistant to both wheat stripe rust and powdery mildew, which are caused by Puccinia striiformis f. sp. tritici (Pst) and Blumeria graminis f. sp. tritici (Bgt), respectively. To explore the difference between wheat defense response against Pst and Bgt, quantitative proteomic analyses of XM318 inoculated with either Pst or Bgt were performed using tandem mass tags technology. A total of 741 proteins were identified as differentially accumulated proteins (DAPs). Bioinformatics analyses indicated that some functional categories, including antioxidant activity and immune system process, exhibited obvious differences between Pst and Bgt infections. Intriguingly, only 42 DAPs responded to both Pst and Bgt infections. Twelve DAPs were randomly selected for reverse transcription quantitative polymerase chain reaction (RT-qPCR) analysis, and the mRNA expression levels of 11 were consistent with their protein expression. Furthermore, gene silencing using the virus-induced gene silencing system indicated that glutathione S-transferase (TaGSTU6) has an important role in resistance to Bgt but not to Pst. TaGSTU6 interacted with the cystathionine beta-synthase (CBS) domain-containing protein (TaCBSX3) in both Pst and Bgt infections. Knockdown of TaCBSX3 expression only reduced wheat resistance to Bgt infection. Overexpression of TaGSTU6 and TaCBSX3 in Arabidopsis (Arabidopsis thaliana) promoted plant resistance to Pseudomonas syringae pv. Tomato DC3000. Our results indicate that TaGSTU6 interaction with TaCBSX3 only confers wheat resistance to Bgt, suggesting that wheat has different response mechanisms to Pst and Bgt stress.

中文翻译：

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谷胱甘肽 S-转移酶相互作用增强小麦对白粉病的抗性，但不能增强小麦条锈病的抗性。

小麦条锈病和白粉病是世界范围内重要的小麦病害。小麦品种兴民318（XM318）对由条锈菌f引起的小麦条锈病和白粉病具有抗性。sp。小麦 (Pst) 和 Blumeria graminis f. sp。小麦（Bgt），分别。为了探索小麦针对 Pst 和 Bgt 的防御反应之间的差异，使用串联质量标签技术对接种 Pst 或 Bgt 的 XM318 进行定量蛋白质组分析。总共 741 个蛋白质被鉴定为差异累积蛋白质 (DAP)。生物信息学分析表明，Pst 和 Bgt 感染之间的一些功能类别，包括抗氧化活性和免疫系统过程，表现出明显的差异。有趣的是，只有 42 个 DAP 对 Pst 和 Bgt 感染都有反应。随机选择12个DAP进行逆转录定量聚合酶链反应（RT-qPCR）分析，11个DAP的mRNA表达水平与其蛋白表达一致。此外，使用病毒诱导的基因沉默系统进行的基因沉默表明，谷胱甘肽S-转移酶（TaGSTU6）在抗Bgt方面具有重要作用，但对Pst没有作用。在 Pst 和 Bgt 感染中，TaGSTU6 与含胱硫醚 β-合酶 (CBS) 结构域的蛋白 (TaCBSX3) 相互作用。TaCBSX3 表达的敲低仅降低了小麦对 Bgt 感染的抗性。TaGSTU6 和 TaCBSX3 在拟南芥 (Arabidopsis thaliana) 中的过表达促进了植物对丁香假单胞菌 (Pseudomonas syringae pv.) 的抗性。番茄DC3000。我们的结果表明，TaGSTU6 与 TaCBSX3 的相互作用仅赋予小麦对 Bgt 的抗性，这表明小麦对 Pst 和 Bgt 胁迫具有不同的响应机制。