Natural products (+)-nootkatone is an important sesquiterpene compound and is widely used in pharmaceutical, cosmetic, agricultural and food industries. The aim of this study was to analyze the differentially expressed proteins (DEPs) during citrus aroma compound (+)-valencene biotransformation to (+)-nootkatone by Yarrowia lipolyticaby with high-throughput LC-MS/MS. A total of 778 proteins were differentially expressed, 385 DEPs were significantly up-regulated and 393 DEPs were markedly down-regulated. It was found that the enzymes transformed (+)-valencene to (+)-nootkatone were mainly existed in yeast intracellular and precipitated under the condition of 30–40 % ammonium sulfate. Most DEPs involved in amino acid and fatty acid metabolism were down-regulated during (+)-valencene biotransformation. The DEPs related to the carbohydrate metabolism, energy metabolism and most of transporter proteins were significantly up-regulated. Furthermore, the key enzymes involved in (+)-valencene transformation might be related to cytochrome P450s (gene2215 and gene2911) and dehydrogenases (gene6493). This is the first time that proteomics was used to investigate the metabolism mechanism of Yarrowia lipolytica during (+)-valencene biotransformation. The proteomic analysis of Yarrowia lipolytica provided a foundation for the molecular regulatory mechanism in the biotransformation to (+)-nootkatone from (+)-valencene.

天然产物 (+)-nootkatone 是一种重要的倍半萜类化合物，广泛用于制药、化妆品、农业和食品工业。本研究的目的是分析解脂耶氏酵母 ( Yarrowia lipolyticaby ) 将柑橘香气化合物 (+)-valencene 生物转化为 (+)-nootkatone 过程中的差异表达蛋白 (DEPs)采用高通量 LC-MS/MS。共有778个蛋白质差异表达，385个DEPs显着上调，393个DEPs显着下调。发现将(+)-瓦伦烯转化为(+)-诺卡酮的酶主要存在于酵母细胞内，并在30-40%硫酸铵条件下沉淀。大多数参与氨基酸和脂肪酸代谢的 DEP 在 (+)-瓦伦烯生物转化过程中被下调。与碳水化合物代谢、能量代谢和大部分转运蛋白相关的DEPs显着上调。此外，参与（+）-瓦伦烯转化的关键酶可能与细胞色素 P450（基因 2215 和基因 2911）和脱氢酶（基因 6493）有关。这是第一次使用蛋白质组学研究蛋白质的代谢机制。(+)-瓦伦烯生物转化过程中的解脂耶氏酵母。解脂耶氏酵母的蛋白质组学分析为(+)-瓦伦烯生物转化为(+)-nootkatone的分子调控机制提供了基础。