Biochar Effectively Inhibits the Horizontal Transfer of Antibiotic Resistance Genes via Restraining the Energy Supply for Conjugative Plasmid Transfer,Environmental Science & Technology

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Biochar Effectively Inhibits the Horizontal Transfer of Antibiotic Resistance Genes via Restraining the Energy Supply for Conjugative Plasmid Transfer
Environmental Science & Technology ( IF 10.8 ) Pub Date : 2022-08-09 , DOI: 10.1021/acs.est.2c02701
Yajing Wu _{1,

2} , Huicong Yan _{1,

2} , Xiaomin Zhu ₃ , Congcong Liu _{1,

2} , Chiheng Chu _{1,

2} , Xiaoying Zhu _{1,

2} , Baoliang Chen _{1,

2}

Affiliation

Horizontal gene transfer (HGT) of antibiotic resistance genes (ARGs) through plasmid-mediated conjugation poses a major threat to global public health. Biochar, a widely used environmental remediation material, has remarkable impacts on the fate of ARGs. However, although biochar was reported being able to inhibit the HGT of ARGs via conjugation and transformation, little is known about the intracellular process that mediates the inhibition effects. On the other hand, as typical natural organic matter, fulvic acid is a common environmental influencer, and how it interferes with the effect of biochar on the HGT of ARGs is unknown. Therefore, this study investigated the effects on the conjugative transfer of ARGs between Escherichia coli MG1655 and E. coli HB101 carrying plasmid RP4, with biochars pyrolyzed at three temperatures and with the corresponding biochars coating with fulvic acid. Results showed that biochar with higher pyrolyzed temperature had a more substantial inhibitory effect on the conjugative transfer of the RP4 plasmid. The inhibitory effect of biochar was mainly attributed to (i) down-regulation of plasmid transfer gene expression, including the formation of conjugative transfer channel and plasmid replication, due to restrained adenosine triphosphate (ATP) energy supply and (ii) decreased cell membrane permeability. Conversely, the fulvic acid coating diminished this inhibition effect of biochar, mainly by providing more ATP and strengthening intracellular reactive oxygen species (ROS) defense. Our findings shed light on the intracellular process that mediates the effects of biochar on the conjugative transfer of ARGs, which would provide support for using biochar to reduce the spread of ARGs.

中文翻译：

Biochar 通过抑制结合质粒转移的能量供应，有效抑制抗生素耐药基因的水平转移

通过质粒介导的偶联对抗生素抗性基因 (ARGs) 进行水平基因转移 (HGT) 对全球公共卫生构成重大威胁。生物炭是一种广泛使用的环境修复材料，对 ARGs 的命运有着显着的影响。然而，尽管据报道 biochar 能够通过缀合和转化抑制 ARGs 的 HGT，但对介导抑制作用的细胞内过程知之甚少。另一方面，作为典型的天然有机物，黄腐酸是一种常见的环境影响因素，其如何干扰生物炭对ARGs HGT的影响尚不清楚。因此，本研究调查了 ARGs 在大肠杆菌MG1655 和大肠杆菌之间的结合转移的影响。HB101 携带质粒 RP4，生物炭在三个温度下热解，相应的生物炭涂有富里酸。结果表明，热解温度较高的生物炭对RP4质粒的结合转移具有更显着的抑制作用。biochar 的抑制作用主要归因于 (i) 由于抑制三磷酸腺苷 (ATP) 能量供应和 (ii) 细胞膜通透性降低，质粒转移基因表达的下调，包括结合转移通道的形成和质粒复制. 相反，富里酸涂层降低了生物炭的这种抑制作用，主要是通过提供更多的 ATP 和加强细胞内活性氧 (ROS) 防御。

更新日期：2022-08-09

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