One of the hallmarks of plant senescence is the global transcriptional reprogramming coordinated by a plethora of transcription factors (TFs). However, mechanisms underlying the interactions between different TFs in modulating senescence remain obscure. Previously, we discovered that plant ABS3 subfamily MATE transporter genes regulate senescence and senescence-associated transcriptional changes. In a genetic screen for mutants suppressing the accelerated senescence phenotype of the gain-of-function mutant abs3-1D, AUXIN RESPONSE FACTOR 2 (ARF2) and PHYTOCHROME-INTERACTING FACTOR 5 (PIF5) were identified as key TFs responsible for transcriptional regulation in the ABS3-mediated senescence pathway. ARF2 and PIF5 (as well as PIF4) interact directly and function interdependently to promote senescence, and they share common target genes such as key senescence promoting genes ORESARA 1 (ORE1) and STAY-GREEN 1 (SGR1) in the ABS3-mediated senescence pathway. In addition, we discovered reciprocal regulation between ABS3-subfamily MATEs and the ARF2 and PIF5/4 TFs. Taken together, our findings reveal a regulatory paradigm in which the ARF2-PIF5/4 functional module facilitates the transcriptional reprogramming in the ABS3-mediated senescence pathway.

中文翻译：

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ARF2-PIF5相互作用控制ABS3介导的植物衰老途径中的转录重编程

植物衰老的标志之一是由大量转录因子（TF）协调的全局转录重编程。然而，不同转录因子之间相互作用调节衰老的机制仍然不清楚。此前，我们发现植物ABS3亚家族MATE转运蛋白基因调节衰老和衰老相关的转录变化。在对抑制功能获得突变体abs3-1D加速衰老表型的突变体的遗传筛选中，生长素反应因子2（ARF2）和植物色素相互作用因子5（PIF5）被确定为负责转录调控的关键转录因子。 ABS3 介导的衰老途径。ARF2 和 PIF5（以及 PIF4）直接相互作用并相互依赖地促进衰老，并且它们具有共同的靶基因，例如ABS3 介导的衰老途径中的关键衰老促进基因ORESARA 1 ( ORE1 ) 和STAY-GREEN 1 ( SGR1 ) 。此外，我们发现 ABS3 亚家族 MATE 与 ARF2 和 PIF5/4 TF 之间存在相互调节。总而言之，我们的研究结果揭示了一种调节范式，其中 ARF2-PIF5/4 功能模块促进 ABS3 介导的衰老途径中的转录重编程。