Telocytes (TCs) are a vital constituent of interstitial tissue. They contribute to regulating cell function in heterotypic connections via direct contact or paracrine singling. Few studies mentioned intraepithelial TCs; however, they have been identified with scanning electron microscopy (SEM) and transmission electron microscopy (TEM). In this study, we investigated the intraepithelial and interstitial TCs using immunohistochemistry (IHC) and TEM. TCs can be identified by their distinctive telopodes (TPs), which consist of podoms and podomere, using TEM and immunohistochemical staining with CD34, CD117, and VEGF antibodies. Intraepithelial TCs established heterocontact with the lamellar capillary and interstitial TCs connected with the blood vessel in lamina propria. Intraepithelial TCs established direct contact with epithelial cells, which formed the lymph space while interstitial TCs connected with the secondary vascular vessels. The study provides evidence for TCs' heterocontact with lamellar blood capillaries, the blood vessels, chloride cells, and immune cells, such as rodlet cells and lymphocytes. In conclusion, TCs have a role in regulating respiratory activities, maintaining osmotic pressure, modulating the immune response, and conducting immunosurveillance.

中文翻译：

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鱼鳃中两种不同类型的细胞：光、免疫组织化学和超微结构研究

端粒细胞 (TC) 是间质组织的重要组成部分。它们通过直接接触或旁分泌单选有助于调节异型连接中的细胞功能。很少有研究提到上皮内 TC；然而，它们已通过扫描电子显微镜 (SEM) 和透射电子显微镜 (TEM) 进行鉴定。在这项研究中，我们使用免疫组织化学 (IHC) 和 TEM 研究了上皮内和间质 TC。TCs 可以通过其独特的端足 (TPs) 来识别，TPs 由豆荚和豆粒组成，使用 TEM 和用 CD34、CD117 和 VEGF 抗体进行免疫组织化学染色。上皮内 TCs 与层状毛细血管和间质 TCs 与固有层中的血管建立异质接触。上皮内 TCs 与上皮细胞直接接触，其形成淋巴空间，而间质TC与次级血管相连。该研究为 TCs 与层状毛细血管、血管、氯化物细胞和免疫细胞（如棒状细胞和淋巴细胞）的异质接触提供了证据。总之，TC在调节呼吸活动、维持渗透压、调节免疫反应和进行免疫监视方面具有作用。