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Construction of magnetic covalent organic frameworks functionalized by benzoboroxole for efficient enrichment of glycoproteins in the physiological environment
Talanta ( IF 5.6 ) Pub Date : 2022-08-08 , DOI: 10.1016/j.talanta.2022.123772
Jingyi Luan 1 , Xinkui Zhu 1 , Licheng Yu 1 , Yijun Li 2 , Xiwen He 1 , Langxing Chen 1 , Yukui Zhang 3
Affiliation  

To date, the development of highly selective and efficient glycoproteins/peptides enrichment is still a challenge for mass spectrometry-based proteomic analysis. In this work, we reported a novel strategy to prepare a magnetic amide-linked covalent organic framework functionalized by benzoboroxole (denoted as Fe3O4@COF-ABB), which was then used as an adsorbent for the enrichment of glycoproteins. The physical and adsorption properties of Fe3O4@COF-ABB were fully investigated. The Fe3O4@COF-ABB presents a regular core-shell spherical structure, quick magnetic response performance, regular porosity, and multiple binding sites of phenylboronic acid. Taking advantage of these benefits, the synthesized magnetic composites exhibited a superior adsorption capacity (565.8 mg g−1) and high selectivity towards glycoprotein immunoglobulin G (IgG) under physiological state (pH 7.4). Additionally, the adsorbent Fe3O4@COF-ABB could be easily regenerated and reused 5 times with no reduction of enrichment performance. More importantly, the practical applications of Fe3O4@COF-ABB were further demonstrated by the selective adsorption of IgG from human serum. The present work represents a rational design of versatile functionalization of magnetic COFs, which demonstrates an avenue for the selective enrichment and analysis of IgG from real biological sample matrices.



中文翻译:

构建由苯并硼氧烷功能化的磁性共价有机框架,用于在生理环境中有效富集糖蛋白

迄今为止,开发高选择性和高效的糖蛋白/肽富集仍然是基于质谱的蛋白质组学分析的挑战。在这项工作中,我们报道了一种新的策略来制备由苯并硼氧烷功能化的磁性酰胺连接的共价有机框架(表示为 Fe 3 O 4 @COF-ABB),然后将其用作糖蛋白富集的吸附剂。对 Fe 3 O 4 @COF-ABB的物理和吸附性能进行了充分研究。Fe 3 O 4@COF-ABB 呈现规则的核壳球形结构、快速的磁响应性能、规则的孔隙率和苯硼酸的多个结合位点。利用这些优点,合成的磁性复合材料在生理状态(pH 7.4)下表现出优异的吸附能力(565.8 mg g -1 )和对糖蛋白免疫球蛋白G(IgG)的高选择性。此外,吸附剂 Fe 3 O 4 @COF-ABB 可以很容易地再生和重复使用 5 次,而不会降低富集性能。更重要的是,Fe 3 O 4的实际应用@COF-ABB 通过从人血清中选择性吸附 IgG 得到进一步证明。目前的工作代表了磁性 COF 多功能功能化的合理设计,它展示了从真实生物样品基质中选择性富集和分析 IgG 的途径。

更新日期:2022-08-12
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