Developing wearable flexible biosensors with excellent electrical conductivity and outstanding flexibility simultaneously for disease diagnosis and health monitoring is a current hot topic in the field of sensor research. In this study, nitrogen-doped graphene (N-Gr) and gold nanoparticles (AuNPs) were successively deposited onto ITO-PET flexible conductive films by chemical deposition, and then a flexible electrochemical immunosensor with high sensitivity and selectivity was constructed for detecting depression markers by exploiting the high affinity between AuNPs and the sulfhydryl groups of depression marker (DM) antibody. Due to the composite of N-Gr, AuNPs and ITO-PET, the prepared flexible sensor can maintain a relatively stable electrical signal response regardless of the deformation such as spiral, rolling, and bending, without shedding or fracture of the N-Gr and AuNPs, and can also significantly amplify the electrochemical response signal of the immunosensor. Under optimized experimental conditions, the fabricated immunosensor showed good linearity over a wide range of 0.023–300.00 ng/mL with a low detection limit of 0.010 ng/mL (3σ, n = 5), when it was used for the determination of depression markers-human Apo-A4 in 100% whole serum samples. The flexibility of the constructed immunosensor and the stability and sensitivity of biomolecular analysis are expected to be further made into implantable depression marker in-situ monitoring probe with the help of micro-machining technology, which will develop a promising method for objective, efficient and accurate clinical diagnosis of depression.

开发同时具有出色导电性和出色灵活性的可穿戴柔性生物传感器用于疾病诊断和健康监测是当前传感器研究领域的热门话题。本研究通过化学沉积法将氮掺杂石墨烯（N-Gr）和金纳米粒子（AuNPs）依次沉积在ITO-PET柔性导电薄膜上，构建了一种高灵敏度、高选择性的柔性电化学免疫传感器，用于检测抑郁症标志物。通过利用 AuNPs 和抑郁标记 (DM) 抗体的巯基之间的高亲和力。由于 N-Gr、AuNPs 和 ITO-PET 的复合，所制备的柔性传感器可以在不受螺旋、滚动和弯曲等变形的影响下保持相对稳定的电信号响应，N-Gr和AuNPs不会脱落或断裂，并且还可以显着放大免疫传感器的电化学响应信号。在优化的实验条件下，制备的免疫传感器在0.023-300.00 ng/mL的宽范围内表现出良好的线性，低检测限为0.010 ng/mL（3σ，n = 5），用于抑郁症标志物的测定- 100% 全血清样品中的人 Apo-A4。所构建的免疫传感器的灵活性和生物分子分析的稳定性和灵敏度，有望在微加工技术的帮助下进一步制成植入式凹陷标记原位监测探针，为客观、高效、准确的检测方法开辟一条前景广阔的道路。抑郁症的临床诊断。还可以显着放大免疫传感器的电化学响应信号。在优化的实验条件下，制备的免疫传感器在0.023-300.00 ng/mL的宽范围内表现出良好的线性，低检测限为0.010 ng/mL（3σ，n = 5），用于抑郁症标志物的测定- 100% 全血清样品中的人 Apo-A4。所构建的免疫传感器的灵活性和生物分子分析的稳定性和灵敏度，有望在微加工技术的帮助下进一步制成植入式凹陷标记原位监测探针，为客观、高效、准确的检测方法开辟一条前景广阔的道路。抑郁症的临床诊断。还可以显着放大免疫传感器的电化学响应信号。在优化的实验条件下，制备的免疫传感器在0.023-300.00 ng/mL的宽范围内表现出良好的线性，低检测限为0.010 ng/mL（3σ，n = 5），用于抑郁症标志物的测定- 100% 全血清样品中的人 Apo-A4。所构建的免疫传感器的灵活性和生物分子分析的稳定性和灵敏度，有望在微加工技术的帮助下进一步制成植入式凹陷标记原位监测探针，为客观、高效、准确的检测方法开辟一条前景广阔的道路。抑郁症的临床诊断。制备的免疫传感器在用于抑郁症标志物-人 Apo-A4 的测定时，在 0.023-300.00 ng/mL 的宽范围内表现出良好的线性，低检测限为 0.010 ng/mL (3σ, n = 5)在 100% 全血清样本中。所构建的免疫传感器的灵活性和生物分子分析的稳定性和灵敏度，有望在微加工技术的帮助下进一步制成植入式凹陷标记原位监测探针，为客观、高效、准确的检测方法开辟一条前景广阔的道路。抑郁症的临床诊断。制备的免疫传感器在用于抑郁症标志物-人 Apo-A4 的测定时，在 0.023-300.00 ng/mL 的宽范围内表现出良好的线性，低检测限为 0.010 ng/mL (3σ, n = 5)在 100% 全血清样本中。所构建的免疫传感器的灵活性和生物分子分析的稳定性和灵敏度，有望在微加工技术的帮助下进一步制成植入式凹陷标记原位监测探针，为客观、高效、准确的检测方法开辟一条前景广阔的道路。抑郁症的临床诊断。