The FungalRoot database aims to equip scientists with correct and comprehensive information about arbuscular mycorrhizal (AM), ectomycorrhizal (EcM) and ericoid mycorrhizal (ErM) associations between plants and fungi compiled from original research papers (Soudzilovskaia et al., 2020). In this article, we introduce a new release of the FungalRoot (v.2.0) online database of plant mycorrhizal associations and provide a response to the Letter by Bueno et al. (2021) that questions protocols used in the assembly of v.1.0 of FungalRoot (Soudzilovskaia et al., 2020).

FungalRoot extends the state-of-the-art of mycorrhiza data collections by including a method of determination, number of replicates, and metadata about plant and soil contexts of root mycorrhizal colonization. In FungalRoot v.2.0, we have expanded the possibilities for third-party opinion and justification where conflicting mycorrhiza assignments occur. The new field ‘arbuscule presence confirmed in follow-up studies’ flags records of arbuscules in roots. We invite colleagues to provide similar assessments based on other criteria of AM symbiosis, and have enabled our database to be expanded with such assessments.

The authors of many original studies have not provided exact definitions of the mycorrhizas used in their studies. Although we promote combined structural, functional and taxonomic definitions of mycorrhiza types in new diagnostic studies of mycorrhiza (Brundrett & Tedersoo, 2018; Soudzilovskaia et al., 2020), it is often impossible to apply these criteria to pre-existing data. To maximize the use of historical data in FungalRoot (both versions), we have presented the original assignments of mycorrhiza types and specified the assessment method. FungalRoot v.2.0 also includes new species-by-site records from recent publications (2019–2021). Collectively, FungalRoot represents the largest empirical database of plant mycorrhizal associations world-wide, containing 36 545 species-by-site records.

FungalRoot 数据库旨在为科学家提供有关植物与真菌之间的丛枝菌根 (AM)、外生菌根 (EcM) 和石蜡菌根 (ErM) 关联的正确和全面的信息，这些关联来自原始研究论文（Soudzilovskaia等人，2020 年）。在本文中，我们介绍了植物菌根关联的 FungalRoot (v.2.0) 在线数据库的新版本，并提供了对Bueno等人的来信的回应。（2021 年）质疑 FungalRoot v.1.0 组装中使用的协议（Soudzilovskaia等人，2020 年）。

FungalRoot 扩展了最先进的菌根数据收集，包括确定方法、重复次数以及有关根菌根定植的植物和土壤背景的元数据。在 FungalRoot v.2.0 中，我们扩大了第三方意见的可能性和发生菌根分配冲突的理由。新领域“在后续研究中证实的丛枝存在”标志着根中丛枝的记录。我们邀请同事根据 AM 共生的其他标准提供类似的评估，并使我们的数据库能够通过此类评估进行扩展。

许多原始研究的作者没有提供他们研究中使用的菌根的确切定义。尽管我们在菌根的新诊断研究中提倡菌根类型的组合结构、功能和分类定义（Brundrett & Tedersoo，2018 年；Soudzilovskaia等人，2020 年）)，通常不可能将这些标准应用于预先存在的数据。为了最大限度地利用FungalRoot（两个版本）中的历史数据，我们给出了菌根类型的原始分配并指定了评估方法。FungalRoot v.2.0 还包括来自最近出版物（2019-2021 年）的新物种逐个记录。总的来说，FungalRoot 代表了世界范围内最大的植物菌根协会经验数据库，包含 36 545 个不同地点的物种记录。