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Optical Phase Contrast Microscopy with Incoherent Vortex Phase
Laser & Photonics Reviews ( IF 9.8 ) Pub Date : 2022-08-07 , DOI: 10.1002/lpor.202200230 Mengting Zhao 1 , Xinzhou Liang 1 , Jiasui Li 1 , Mengyuan Xie 1 , Huadan Zheng 1 , Yongchun Zhong 2 , Jianhui Yu 2 , Jun Zhang 1 , Zhe Chen 1 , Wenguo Zhu 1
Laser & Photonics Reviews ( IF 9.8 ) Pub Date : 2022-08-07 , DOI: 10.1002/lpor.202200230 Mengting Zhao 1 , Xinzhou Liang 1 , Jiasui Li 1 , Mengyuan Xie 1 , Huadan Zheng 1 , Yongchun Zhong 2 , Jianhui Yu 2 , Jun Zhang 1 , Zhe Chen 1 , Wenguo Zhu 1
Affiliation
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A novel, compact, and broadband optical phase contrast microscopy based on incoherent vortex topological quadrupole is theoretically proposed and experimentally realized. The topological quadrupole, generated in a thin uniaxial crystal, possesses four single-charge optical vortexes, each of which can act as a two-dimensional (2D) optical spatial differentiator. The incoherence of light-emitting diode (LED) light will wash out the optical vortex and the spatial differentiation effect, which can be ingeniously overcome by choosing Kohler illumination and inserting a 1 mm-thickness uniaxial crystal sandwiched with two polarizers before the camera. By adjusting the polarizers and the tilted angle of crystal to selectively utilize the geometric Berry phase and the angular gradient of Fresnel transmission coefficients of crystal, the 1D, 2D, and second-order analog spatial differentiations with a spatial resolution better than 0.775 μm can be switched flexibly. A versatile phase contrast microscope is built, which is well compatible with the conventional microscopes. The uniformly incoherent illumination without laser speckle effects results in high-quality spatial differentiation imaging. Biologically transparent living cells are thus imaged with high contrast.
中文翻译：
具有非相干涡旋相位的光学相衬显微镜
理论上提出并实验实现了一种基于非相干涡旋拓扑四极杆的新型紧凑型宽带光学相衬显微镜。在薄单轴晶体中产生的拓扑四极具有四个单电荷光学涡旋，每个涡旋都可以充当二维 (2D) 光学空间微分器。发光二极管 (LED) 光的不相干性会冲淡光学涡旋和空间微分效应，可以通过选择科勒照明并在相机前插入夹有两个偏振片的 1 毫米厚单轴晶体来巧妙地克服这一问题。通过调整偏光片和晶体的倾角，选择性地利用几何Berry相位和晶体菲涅尔透射系数的角梯度，得到1D、2D、空间分辨率优于0.775 μm的二阶模拟空间微分可以灵活切换。构建了一种多功能相差显微镜，与传统显微镜具有良好的兼容性。没有激光散斑效应的均匀非相干照明可实现高质量的空间微分成像。因此，生物透明的活细胞以高对比度成像。
更新日期：2022-08-07
中文翻译：
具有非相干涡旋相位的光学相衬显微镜
理论上提出并实验实现了一种基于非相干涡旋拓扑四极杆的新型紧凑型宽带光学相衬显微镜。在薄单轴晶体中产生的拓扑四极具有四个单电荷光学涡旋，每个涡旋都可以充当二维 (2D) 光学空间微分器。发光二极管 (LED) 光的不相干性会冲淡光学涡旋和空间微分效应，可以通过选择科勒照明并在相机前插入夹有两个偏振片的 1 毫米厚单轴晶体来巧妙地克服这一问题。通过调整偏光片和晶体的倾角，选择性地利用几何Berry相位和晶体菲涅尔透射系数的角梯度，得到1D、2D、空间分辨率优于0.775 μm的二阶模拟空间微分可以灵活切换。构建了一种多功能相差显微镜，与传统显微镜具有良好的兼容性。没有激光散斑效应的均匀非相干照明可实现高质量的空间微分成像。因此，生物透明的活细胞以高对比度成像。
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