Brassica plants play an important role in common agricultural practices, such as livestock feed or biofumigation, due to the bioactivity of the natural degradation products of glucosinolate metabolites. Therefore, the ability to survey comprehensive glucosinolate profiles for individual brassicas is essential for informing proper species selection for the intended application. Current methods for glucosinolate identification and quantification involve complex or unconventional procedures, and proper reference materials are not readily available. Therefore, researchers with limited resources that require glucosinolate profiles are at an extreme disadvantage. In this work, a simple and accurate HPLC-MS method was developed and validated to build preliminary glucosinolate profiles for three agriculturally relevant forage brassica varieties [turnip (B. rapa L.), canola (B. napus L.), and rapeseed (B. napus L.)]. The average glucosinolate content across three herbage collection dates for canola, rapeseed and turnip were 2.9 ± 0.9 mg g⁻¹, 6.4 ± 1.3 mg g⁻¹, and 14 ± 3.4 mg g⁻¹, respectively. GLS concentrations are reported in milligrams of glucosinolate, calculated as sinigrin equivalents, per gram of dry plant material. This semi-quantitative approach for reporting total GLS content in brassicas is accurate within 15%. Several minor individual glucosinolates were identified that have not been previously reported in canola, rapeseed and turnip species, including glucotropaeolin and 4-hydroxyglucobrassicin (canola), glucoraphanin and glucoberteroin (rapeseed), and glucosinalbin and glucobarbarin (turnip). This non-targeted screen of several forage brassica varieties demonstrates the inherent variation in both the individual glucosinolate content and the total glucosinolate profile among brassicas, and highlights the importance of such glucosinolate characterization in agricultural practices. Additionally, the method developed in this study can be used as a tool for researchers with limited resources to build accurate glucosinolate profiles of brassica plants.

由于芥子油苷代谢物的天然降解产物的生物活性，芸苔属植物在常见的农业实践中发挥着重要作用，例如牲畜饲料或生物熏蒸。因此，调查单个芸苔属植物的综合硫代葡萄糖苷概况的能力对于为预期应用提供适当的物种选择至关重要。当前的硫代葡萄糖苷鉴定和定量方法涉及复杂或非常规的程序，并且不容易获得适当的参考材料。因此，需要硫代葡萄糖苷谱的资源有限的研究人员处于极端不利的境地。在这项工作中，开发并验证了一种简单而准确的 HPLC-MS 方法，以建立三种农业相关草料芸苔品种 [芜菁 (B. rapa L.)、油菜 ( B. napus L.) 和油菜 ( B. napus L.)]。油菜籽、油菜籽和萝卜的三个草本采集日期的平均硫代葡萄糖苷含量分别为 2.9 ± 0.9 mg g ^-1、6.4 ± 1.3 mg g ^-1和 14 ± 3.4 mg g ^-1， 分别。GLS 浓度以每克干植物材料中硫代葡萄糖苷的毫克数计算，计算为黑芥子苷当量。这种报告芸苔中总 GLS 含量的半定量方法的准确度在 15% 以内。已鉴定出一些以前在油菜、油菜和萝卜物种中未曾报道过的次要的硫代葡萄糖苷，包括芥子油苷和 4-羟基芥子油菜素（油菜）、萝卜硫素和葡萄糖苷（油菜籽），以及芥子苷和氨基葡萄糖苷（萝卜）。这种对几种草料芸苔品种的非靶向筛选展示了芸苔中单个硫代葡萄糖苷含量和总硫代葡萄糖苷含量的内在差异，并强调了这种硫代葡萄糖苷表征在农业实践中的重要性。此外，