In this paper, chitosan (Chi) was successfully reacted with natural mono ketone namely, camphor (Cam), through a condensation reaction to obtain a novel Chi-Cam polymer. The encapsulation of Chi-Cam polymer into the cavity of β-cyclodextrin biopolymer by inclusion complex method, and the immobilization of TiO₂ NPs into Chi-Cam matrix by metal complex method were performed. A set of characterizations (XRD, FTIR, SEM, UV–Vis, optical band gap, Urbach energy, and refractive index, and thermogravimetric analysis) were implemented to incorporate their properties with applications. It was not observed the existence of any strange peaks in diffractogram patterns of all prepared samples, confirming the proper preparation and high purity polymers. The crystalline index was found to be 69.41, 41.58, 58.25 and 44.2 % for Chi, Chi-Cam, polyrotaxane, and nanocomposite, respectively. High surface area, lowest pores size, and lowest pores volume were confirmed the micropores of composite, which also could provide more reactive sites, and enhancing the bioactivity. The tested Chi-Cam/TiO₂ NPs possessed the highest impact, followed by the Chi-Cam/β-CD towards microbes. The highest % inhibition of biofilm formation by Chi-Cam, Chi-Cam/β-CD, and Chi-Cam/TiO₂ NPs was noted for S. aureus (40.05 %, 80.21 %, and 87.49 %), E. coli (38.56 %, 74.32 % and 83.32 %), P. aureginosa (32.12 %, 58.80 %, and 74.53 %), and C. albicans (13.69 %, 27.89 % and 70.89 %), respectively. The obtaining findings pave the route towards useful utilization of potential materials for biomedical applications.

本文将壳聚糖（Chi）与天然单酮樟脑（Cam）通过缩合反应成功反应得到新型Chi-Cam聚合物。Chi-Cam聚合物通过包合络合法包封到β-环糊精生物聚合物的空腔中，并固定化TiO ₂通过金属络合物法将纳米粒子转化为 Chi-Cam 基质。实施了一组表征（XRD、FTIR、SEM、UV-Vis、光学带隙、Urbach 能量和折射率以及热重分析）以将它们的特性与应用结合起来。在所有制备的样品的衍射图中没有观察到任何奇怪的峰的存在，证实了正确的制备和高纯度聚合物。Chi、Chi-Cam、聚轮烷和纳米复合材料的结晶指数分别为 69.41、41.58、58.25 和 44.2%。证实了复合材料的微孔具有高表面积、最小孔径和最小孔体积，这也可以提供更多的反应位点，提高生物活性。测试的 Chi-Cam/TiO ₂NPs 对微生物的影响最大，其次是 Chi-Cam/β-CD。Chi-Cam、Chi-Cam/β-CD 和 Chi-Cam/TiO ₂ NPs对生物膜形成的抑制百分比最高的是金黄色葡萄球菌（40.05 %、80.21 % 和 87.49 %）、大肠杆菌（ 38.56 %、74.32 % 和 83.32 %)、P. aureginosa (32.12 %、58.80 % 和 74.53 %) 和白色念珠菌(13.69 %、27.89 % 和 70.89 %)。获得的发现为将潜在材料用于生物医学应用铺平了道路。