Individual cells are basic units of life. Despite extensive efforts to characterize the cellular heterogeneity of different organisms, cross-species comparisons of landscape dynamics have not been achieved. Here, we applied single-cell RNA sequencing (scRNA-seq) to map organism-level cell landscapes at multiple life stages for mice, zebrafish and Drosophila. By integrating the comprehensive dataset of > 2.6 million single cells, we constructed a cross-species cell landscape and identified signatures and common pathways that changed throughout the life span. We identified structural inflammation and mitochondrial dysfunction as the most common hallmarks of organism aging, and found that pharmacological activation of mitochondrial metabolism alleviated aging phenotypes in mice. The cross-species cell landscape with other published datasets were stored in an integrated online portal—Cell Landscape. Our work provides a valuable resource for studying lineage development, maturation and aging.

中文翻译：

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在单细胞水平上构建跨物种细胞景观


单个细胞是生命的基本单位。尽管为表征不同生物体的细胞异质性做出了广泛的努力，但景观动力学的跨物种比较尚未实现。在这里，我们应用单细胞 RNA 测序 (scRNA-seq) 绘制了小鼠、斑马鱼和果蝇在多个生命阶段的生物体水平细胞图谱。通过整合 > 260 万个单细胞的综合数据集，我们构建了跨物种细胞景观，并确定了在整个生命周期中变化的特征和共同途径。我们将结构性炎症和线粒体功能障碍确定为生物体衰老的最常见标志，并发现线粒体代谢的药理学激活可减轻小鼠的衰老表型。跨物种细胞景观与其他已发布的数据集存储在一个集成的在线门户——细胞景观中。我们的工作为研究谱系发展、成熟和衰老提供了宝贵的资源。