The excellent integration capability of microfluidic platform provides infinite possibilities for the development of analysis and detection technology. A microfluidic immunoassay platform was designed by combining the screen printing and microfluidic chip manufacturing. Four screen printed electrodes were applied as detection units and were divided into two groups for the generation of both electrochemical and photoelectrochemical signals. The light source for the photoelectrochemical process was achieved by the integration of two LED lamp beads in the 3D printed chip holder. Check valves between the chambers of working units and auxiliary units were exploited for the separated steps of working electrode modification and signal detection. A sandwich-type immunosensing configuration was adoped using raspberry-like Au/PANI@CdS nanocomposites as signal tags. Electrochemical and photoelectrochemical current signals can be detected simultaneously on a multichannel electrochemical workstation. After the optimization of microfluidic parameters and other experimental conditions, the microfluidic immunoassay platform shows good analytical performances for the detection of Cyfra 21-1 in the range of 0.1 pg/mL ∼100 ng/mL, with the PEC detection limit of 0.035 pg/mL and the DPV detection limit of 0.041 pg/mL. This multi-functional high-throughput portable microfluidic immunoassay platform has great application prospect in disease diagnosis and instant detection.

微流控平台优异的集成能力为分析检测技术的发展提供了无限可能。结合丝网印刷和微流控芯片制造设计了一个微流控免疫分析平台。四个丝网印刷电极用作检测单元，并分为两组，用于产生电化学和光电化学信号。光电化学过程的光源是通过在 3D 打印芯片支架中集成两个 LED 灯珠来实现的。工作单元室和辅助单元室之间的止回阀用于工作电极修改和信号检测的分离步骤。使用覆盆子状 Au/PANI@CdS 纳米复合材料作为信号标签掺杂夹心型免疫传感配置。电化学和光电化学电流信号可以在多通道电化学工作站上同时检测。经过微流控参数和其他实验条件的优化，微流控免疫分析平台在0.1 pg/mL ∼100 ng/mL范围内对Cyfra 21-1的检测表现出良好的分析性能，PEC检测限为0.035 pg/ mL，DPV 检测限为 0.041 pg/mL。这种多功能高通量便携式微流控免疫分析平台在疾病诊断和即时检测方面具有很大的应用前景。电化学和光电化学电流信号可以在多通道电化学工作站上同时检测。经过微流控参数和其他实验条件的优化，微流控免疫分析平台在0.1 pg/mL ∼100 ng/mL范围内对Cyfra 21-1的检测表现出良好的分析性能，PEC检测限为0.035 pg/ mL，DPV 检测限为 0.041 pg/mL。这种多功能高通量便携式微流控免疫分析平台在疾病诊断和即时检测方面具有很大的应用前景。电化学和光电化学电流信号可以在多通道电化学工作站上同时检测。经过微流控参数和其他实验条件的优化，微流控免疫分析平台对Cyfra 21-1在0.1 pg/mL ∼100 ng/mL范围内的检测表现出良好的分析性能，PEC检测限为0.035 pg/ mL，DPV 检测限为 0.041 pg/mL。这种多功能高通量便携式微流控免疫分析平台在疾病诊断和即时检测方面具有很大的应用前景。PEC 检测限为 0.035 pg/mL，DPV 检测限为 0.041 pg/mL。这种多功能高通量便携式微流控免疫分析平台在疾病诊断和即时检测方面具有很大的应用前景。PEC 检测限为 0.035 pg/mL，DPV 检测限为 0.041 pg/mL。这种多功能高通量便携式微流控免疫分析平台在疾病诊断和即时检测方面具有很大的应用前景。