Liquid‒liquid interface self-assembly was recently proposed to be an efficient strategy for surface-enhanced Raman scattering (SERS) detection. Traditionally, most of the research focuses on the self-assembly of single morphology and homogeneous materials, which are susceptible to the rotation of materials in the liquid phase due to the interference of Brownian motion and the external environment, causing poor stability. In this work, we introduced ternary Ag–Au–SiO₂ nanoparticles to a liquid‒liquid self-assembled SERS platform, utilizing the differences in hydrophilicity of the materials to achieve oriented interfacial arrays, improving the stability of the self-assembled SERS substrate. To enhance the detection efficiency, a dual nucleic acid amplification strategy was designed to reach ultrasensitive detection of target microRNA 21 with a detection limit of (LOD) of 1.41 aM, providing a foresight for the practicability of the two-phase interfacial SERS platform.

最近有人提出液-液界面自组装是一种有效的表面增强拉曼散射 (SERS) 检测策略。传统上，大部分研究集中在单一形态和均质材料的自组装上，由于布朗运动和外部环境的干扰，容易受到液相中材料旋转的影响，导致稳定性较差。在这项工作中，我们引入了三元 Ag-Au-SiO ₂纳米粒子到液-液自组装SERS平台，利用材料亲水性的差异实现定向界面阵列，提高自组装SERS基底的稳定性。为提高检测效率，设计了双核酸扩增策略，实现了对目标microRNA 21 的超灵敏检测，检测限（LOD）为1.41 aM，为两相界面SERS平台的实用性提供了先见之明。