Biomass ethanol production presents shortcomings related to enzymes cost and efficiency of xylose fermentation. Commercial enzymes used in second-generation ethanol (2GE) plants are produced off-site/offshore, with the additional formulation, transportation and storage costs. Alternatively, lower cost cellulases can be produced on-site. This study focused on the techno-economic evaluation of enzymes produced on-site versus commercial. Another limiting feature of the 2GE technology is the use of the hemicellulose sugars for ethanol fermentation. Thus, this work addressed its use for biogas production. Process systems engineering tools were used to evaluate four different configurations of a 2GE plant annexed to an already existent autonomous distillery. The application of the methodology allowed useful perceptions of the ethanol and biogas production, water and electricity consumption, and economic profitability. The need of a second fungus strain for the on-site enzyme production was identified as the main bottleneck of this cellulase production process, which is an important insight that could guide the future research efforts.

生物质乙醇生产存在与酶成本和木糖发酵效率相关的缺点。第二代乙醇 (2GE) 工厂中使用的商业酶是在场外/离岸生产的，需要额外的配方、运输和储存成本。或者，可以在现场生产成本较低的纤维素酶。这项研究的重点是对现场生产的酶与商业生产的酶进行技术经济评估。2GE 技术的另一个限制特征是使用半纤维素糖进行乙醇发酵。因此，这项工作解决了其在沼气生产中的用途。过程系统工程工具用于评估附属于已经存在的自主酿酒厂的 2GE 工厂的四种不同配置。该方法的应用允许对乙醇和沼气生产、水和电消耗以及经济盈利能力进行有用的了解。现场酶生产需要第二种真菌菌株被确定为该纤维素酶生产过程的主要瓶颈，这是可以指导未来研究工作的重要见解。