To meet the emerging demand of portable and on-site assay, a self-powered and flexible split-type aptasensor was constructed based on an enzymatic biofuel cell (EBFC) by using a flexible glucose oxidase (GOD)/Bi₃Ti₂O₈F/carbon nanotubes-reduced graphene oxide (Bi₃Ti₂O₈F/CNTs-rGO) film as the anode and the aptamers fixed AuNPs/CNTs-rGO film as the selective cathode. Bi₃Ti₂O₈F with H₂O₂ enzyme-mimicking property and three-dimensional structure was used for the immobilization of GOD to improve the performance of the EBFC. The recognition of dibutyl phthalate (DBP) was performed in DBP containing solutions using the aptamer/AuNPs/CNTs-rGO cathode, and the detection was carried out in the other glucose containing solution. The output power density of the EBFC decreased after recognizing DBP because of the hindered electron transfer. The aptasensor responded linearly to dibutyl phthalate (DBP) (1.0 × 10⁻¹⁴ to 1.0 × 10⁻⁹ mol/L) with a detection limit of 1.99 × 10⁻¹⁵ mol/L. The bending and stretching tests didn’t influence the output power density of the EBFC, indicating the stability and flexibility of the aptasensor. This sensor had been successfully applied for water sample analysis. It has the potential to be a useful platform for developing implantable, portable, and miniaturized sensors.

为了满足便携式和现场检测的新兴需求，基于酶生物燃料电池（EBFC），使用柔性葡萄糖氧化酶（GOD）/Bi ₃ Ti ₂ O ₈构建了一种自供电的柔性分体式适体传感器。 F/碳纳米管还原的氧化石墨烯（Bi ₃ Ti ₂ O ₈ F/CNTs-rGO）薄膜作为阳极，适配体固定AuNPs/CNTs-rGO薄膜作为选择性阴极。Bi ₃ Ti ₂ O ₈ F 与 H ₂ O ₂酶模拟特性和三维结构用于固定GOD以提高EBFC的性能。使用适体/AuNPs/CNTs-rGO阴极在含DBP的溶液中进行邻苯二甲酸二丁酯（DBP）的识别，并在另一种含葡萄糖的溶液中进行检测。由于电子转移受阻，识别 DBP 后 EBFC 的输出功率密度降低。^{适体传感器对邻苯二甲酸二丁酯 (DBP) (1.0 × 10 -14}至 1.0 × 10 ^-9 mol/L)呈线性响应，检测限为 1.99 × 10 ^-15摩尔/升。弯曲和拉伸测试不影响EBFC的输出功率密度，表明适体传感器的稳定性和灵活性。该传感器已成功应用于水样分析。它有可能成为开发可植入、便携式和小型化传感器的有用平台。