Chitosan (CS) based nanoparticles simultaneously loaded with (−)-epigallocatechin gallate (EGCG) and ferulic acid (FA) were fabricated via ionic gelation method modified by sodium tripolyphosphate and genipin (G-CS-EGCG-FA NPs). The particle size, morphology, entrapment efficiency, rheological properties, antioxidant and tyrosinase inhibitory activity of NPs were investigated. The G-CS-EGCG-FA NPs exhibited irregular ellipsoidal shape with average diameter of 412.3 nm and high DPPH and ABTS·⁺ scavenging ability. The entrapment efficiency of EGCG and FA in NPs was 46.0 ± 1.3 % and 46.8 ± 1.6 %, respectively. CS-based NPs show no toxic effects on NIH 3 T3 cells and B16-F10 melanoma cells with concentration <200 μg/mL and 25 μg/mL, respectively and the cell viability ranged from 100 % to 118 %. Meanwhile, the oxidative repaired capacity of G-CS-EGCG-FA NPs (200 μg/mL) in H₂O₂-induced cells was over 100 %, higher than that of the same dose of free EGCG or FA. Moreover, the tyrosinase inhibition activity of G-CS-EGCG-FA NPs (25 μg/mL) (84.6 %) was more potent than that of free EGCG (55.3 %), free FA (47.1 %) and kojic acid, indicating the good skin repairing and whitening ability of G-CS-EGCG-FA NPs. Given these results, this research provides new insights for designing novel particles loaded with dual bioactive agents that possess synergistic benefits.

通过三聚磷酸钠和京尼平（G-CS-EGCG-FA NPs）改性的离子凝胶法制备同时负载（-）-表没食子儿茶素没食子酸酯（EGCG）和阿魏酸（FA）的壳聚糖（CS）纳米粒子。研究了纳米粒子的粒径、形貌、包封率、流变学性质、抗氧化和酪氨酸酶抑制活性。G-CS-EGCG-FA NPs呈不规则椭圆形，平均直径为412.3 nm，DPPH和ABTS· ⁺清除能力。EGCG 和 FA 在 NPs 中的包封率分别为 46.0 ± 1.3 % 和 46.8 ± 1.6 %。基于 CS 的 NP 对浓度分别 <200 μg/mL 和 25 μg/mL 的 NIH 3 T3 细胞和 B16-F10 黑色素瘤细胞没有毒性作用，细胞活力范围为 100% 至 118%。_{同时，G-CS-EGCG-FA NPs (200 μg/mL)在H 2} O ₂中的氧化修复能力诱导细胞超过100%，高于相同剂量的游离EGCG或FA。此外，G-CS-EGCG-FA NPs (25 μg/mL) (84.6 %) 的酪氨酸酶抑制活性强于游离 EGCG (55.3 %)、游离 FA (47.1 %) 和曲酸，表明G-CS-EGCG-FA NPs具有良好的皮肤修复和美白能力。鉴于这些结果，这项研究为设计装载具有协同作用的双重生物活性剂的新型颗粒提供了新的见解。