The present work aims at evaluating the in vitro biocompatibility, antibacterial activity and antioxidant capacity of the fabricated and optimized Alginate/Chitosan nanoparticles (ALG/CSNPs) and quercetin loaded Alginate/Chitosan nanoparticles (Q-ALG/CSNPs) with an improved biological efficacy on the hydrophobic flavonoid.The physicochemical properties were determined by TEM and FTIR analysis. The nanoparticles evaluated for the encapsulation of quercetin exerted % encapsulation efficiency (EE) that varied between 76 and 82.4 % and loading capacity (LC) from 31 to 46.5 %. Potential cytotoxicity of the ALG/CSNPs and Q-ALG/CSNPs upon L929 fibroblast cell line was evaluated by MTT reduction Assay and expressed as % cell viability. The in vitro antibacterial property was studied by well diffusion method against gram-positive bacteria Staphylococcus aureus (ATCC 25925) and gram-negative bacteria Escherichia coli (ATCC 25923). The inhibitory efficacy by scavenging free radical intermediates was evaluated by 1,1, diphenyl 2-picrylhydrazyl (DPPH) assay. The results of in vitro cytotoxicity showed biocompatibility towards L929 cells. Quercetin loaded Alginate/Chitosan nanoparticles inhibited the growth of microorganisms than pure quercetin. The 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging results have shown a high level of antioxidant property for encapsulated Quercetin in Alginate/Chitosan nanoparticles compared to free Quercetin. The findings of our study suggest that the developed ALG/CSNPs and Q-ALG/CSNPs possess the prerequisites and be proposed as a suitable system for delivering quercetin with enhanced therapeutic effectuality.

目前的工作旨在评估制造和优化的海藻酸盐/壳聚糖纳米粒子（ALG/CSNPs）和槲皮素负载的海藻酸盐/壳聚糖纳米粒子（Q-ALG/CSNPs）的体外生物相容性、抗菌活性和抗氧化能力。疏水性黄酮类化合物。通过TEM和FTIR分析确定了物理化学性质。评估槲皮素包封的纳米颗粒的包封率 (EE) 在 76% 和 82.4% 之间变化，负载能力 (LC) 在 31% 到 46.5% 之间变化。ALG/CSNPs 和 Q-ALG/CSNPs 对 L929 成纤维细胞系的潜在细胞毒性通过 MTT 还原测定法进行评估，并表示为 % 细胞活力。体外的采用微孔扩散法研究了对革兰氏阳性菌金黄色葡萄球菌（ATCC 25925）和革兰氏阴性菌大肠杆菌（ATCC 25923）的抗菌性能。通过 1,1, 二苯基 2-苦基肼 (DPPH) 测定评估清除自由基中间体的抑制效果。体外实验结果细胞毒性显示出对 L929 细胞的生物相容性。负载槲皮素的海藻酸盐/壳聚糖纳米颗粒比纯槲皮素抑制微生物的生长。1,1-二苯基-2-苦基肼 (DPPH) 自由基清除结果表明，与游离槲皮素相比，海藻酸盐/壳聚糖纳米颗粒中包封的槲皮素具有高水平的抗氧化性能。我们的研究结果表明，开发的 ALG/CSNPs 和 Q-ALG/CSNPs 具有先决条件，并被提议作为提供具有增强治疗效果的槲皮素的合适系统。