Simultaneous Monitoring of the Adenosine Triphosphate Levels in the Cytoplasm and Nucleus of a Single Cell with a Single Nanowire-Based Fluorescent Biosensor,Analytical Chemistry

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Simultaneous Monitoring of the Adenosine Triphosphate Levels in the Cytoplasm and Nucleus of a Single Cell with a Single Nanowire-Based Fluorescent Biosensor
Analytical Chemistry ( IF 6.7 ) Pub Date : 2022-08-04 , DOI: 10.1021/acs.analchem.2c02030
Mingliang Mei _{1,

2} , Lixuan Mu ₁ , Yuan Wang _{1,

2} , Sen Liang _{1,

2} , Qiaowen Zhao _{1,

2} , Lushan Huang _{1,

2} , Guangwei She ₁ , Wensheng Shi _{1,

2,

3}

Affiliation

Simultaneous monitoring of the ATP levels at various sites of a single cell is crucial for revealing the ATP-related processes and diseases. In this work, we rationally fabricated single nanowire-based fluorescence biosensors, by which the ATP levels of the cytoplasm and nucleus in a single cell can be simultaneously monitored with a high spatial resolution. Utilizing the as-fabricated single nanowire biosensor, we demonstrated that the ATP levels of the cytoplasm were around 20–30% lower than that of the nucleus in both L929 and HeLa cells. Observing the ATP fluctuation of the cytoplasm and nucleus of the L929 and HeLa cells stimulated by Ca²⁺, oligomycin, or under cisplatin-induced apoptosis, we found that the ATP levels at two cellular sites exhibited discriminative changes, revealing the different mechanisms of the ATP at these two cellular sites in response to the stimulations.

中文翻译：

使用单个基于纳米线的荧光生物传感器同时监测单个细胞的细胞质和细胞核中的三磷酸腺苷水平

同时监测单个细胞不同部位的 ATP 水平对于揭示 ATP 相关过程和疾病至关重要。在这项工作中，我们合理地制造了基于单根纳米线的荧光生物传感器，通过该传感器可以以高空间分辨率同时监测单个细胞中细胞质和细胞核的 ATP 水平。利用制造的单纳米线生物传感器，我们证明了在 L929 和 HeLa 细胞中，细胞质的 ATP 水平比细胞核的 ATP 水平低约 20-30%。观察Ca ^{2+刺激的L929和HeLa细胞细胞质和细胞核的ATP波动}，寡霉素，或在顺铂诱导的细胞凋亡下，我们发现两个细胞位点的ATP水平表现出区别性变化，揭示了这两个细胞位点的ATP响应刺激的不同机制。

更新日期：2022-08-04

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