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Optically Triggering and Monitoring Single-Cell-Level Metabolism Using Ormosil-Decorated Ultrathin Fibers
Langmuir ( IF 3.7 ) Pub Date : 2022-08-04 , DOI: 10.1021/acs.langmuir.2c00915 Chenghu Dai 1, 2 , Hao Jia 1 , Wubin Wu 1 , Baipeng Yin 1, 3 , Hong Wang 1, 3 , Ling Wang 1, 3 , Yeteng Zhong 4 , Zihua Wang 5 , Chuang Zhang 1 , Jiannian Yao 1
Langmuir ( IF 3.7 ) Pub Date : 2022-08-04 , DOI: 10.1021/acs.langmuir.2c00915 Chenghu Dai 1, 2 , Hao Jia 1 , Wubin Wu 1 , Baipeng Yin 1, 3 , Hong Wang 1, 3 , Ling Wang 1, 3 , Yeteng Zhong 4 , Zihua Wang 5 , Chuang Zhang 1 , Jiannian Yao 1
Affiliation
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The integration of biological components and artificial devices requires a bio–machine interface that can simultaneously trigger and monitor the activities in biosystems. Herein, we use an organically modified silicate (ormosil) composite coating containing a light-responsive nanocapsule and a fluorescent bioprobe for reactive oxygen species (ROS) to decorate ultrathin optical fibers, namely, ormosil-decorated ultrathin fibers (OD-UFs), and demonstrate that these OD-UFs can optically trigger and monitor the intracellular metabolism activities in living cells. The sizes and shapes of UF tips were finely controlled to match the dimension and mechanical properties of living cells. The increased elasticity of the ormosil coating of OD-UFs reduces possible mechanical damage during the cell membrane penetration. The light-responsive nanocapsule was physically absorbed on the surface of the ormosil coating and could release a stimulant to trigger the metabolism activities in cells upon the guided laser through OD-UFs. The fluorescent bioprobe was covalently linked with the ormosil matrix for monitoring the intracellular ROS generation, which was verified by the in vitro experiments on the microdroplets of a hydrogen peroxide solution. Finally, we found that the living cells could maintain most of their viability after being inserted with OD-UFs, and the intracellular metabolism activities were successfully triggered and monitored at the single-cell level. The OD-UF provides a new platform for the investigation of intracellular behaviors for drug stimulations and represents a new proof of concept for a bio–machine interface based on the optical and chemical activities of organic functional molecules.
中文翻译：
使用 Ormosil 装饰的超细纤维光学触发和监测单细胞水平的代谢
生物组件和人工设备的集成需要一个能够同时触发和监测生物系统活动的生物机接口。在此，我们使用含有光响应纳米胶囊和活性氧（ROS）荧光生物探针的有机改性硅酸盐（ormosil）复合涂层来装饰超细光纤，即ormosil装饰的超细纤维（OD-UFs），以及证明这些 OD-UFs 可以光学触发和监测活细胞中的细胞内代谢活动。UF 尖端的尺寸和形状经过精细控制，以匹配活细胞的尺寸和机械性能。OD-UFs 的 ormosil 涂层增加的弹性减少了细胞膜穿透过程中可能的机械损伤。光响应纳米胶囊被物理吸收在 ormosil 涂层的表面上，并且可以通过 OD-UFs 在引导激光下释放刺激物以触发细胞内的代谢活动。荧光生物探针与 ormosil 基质共价连接，用于监测细胞内 ROS 的产生。过氧化氢溶液微滴的体外实验。最后，我们发现活细胞在插入 OD-UFs 后可以保持大部分活力，并且在单细胞水平上成功触发和监测细胞内代谢活动。OD-UF 为研究药物刺激的细胞内行为提供了一个新平台，并代表了基于有机功能分子的光学和化学活性的生物机界面的新概念证明。
更新日期：2022-08-04
中文翻译：
使用 Ormosil 装饰的超细纤维光学触发和监测单细胞水平的代谢
生物组件和人工设备的集成需要一个能够同时触发和监测生物系统活动的生物机接口。在此，我们使用含有光响应纳米胶囊和活性氧（ROS）荧光生物探针的有机改性硅酸盐（ormosil）复合涂层来装饰超细光纤，即ormosil装饰的超细纤维（OD-UFs），以及证明这些 OD-UFs 可以光学触发和监测活细胞中的细胞内代谢活动。UF 尖端的尺寸和形状经过精细控制，以匹配活细胞的尺寸和机械性能。OD-UFs 的 ormosil 涂层增加的弹性减少了细胞膜穿透过程中可能的机械损伤。光响应纳米胶囊被物理吸收在 ormosil 涂层的表面上，并且可以通过 OD-UFs 在引导激光下释放刺激物以触发细胞内的代谢活动。荧光生物探针与 ormosil 基质共价连接，用于监测细胞内 ROS 的产生。过氧化氢溶液微滴的体外实验。最后，我们发现活细胞在插入 OD-UFs 后可以保持大部分活力，并且在单细胞水平上成功触发和监测细胞内代谢活动。OD-UF 为研究药物刺激的细胞内行为提供了一个新平台，并代表了基于有机功能分子的光学和化学活性的生物机界面的新概念证明。
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