Photodynamic therapy (PDT) is a promising cancer treatment with fewer side effects, and it eliminates tumors in target tissues with reactive oxygen species produced by photosensitizers (PS) and light. In this study, we isolated methyl pheophorbide A, which induces photodynamic cell death in the U937 and SK-HEP-1 cells, from perilla leaves. Its potential as a material for the development of a new PS was also evaluated. The methyl pheophorbide A is a dark green porphyrin compound isolated from methanol extract of perilla leaves. Apoptosis occurred was methyl pheophorbide A treated and irradiated with light, and there was no significant change where light not treated. In both U937 and SK-HEP-1 cells, apoptotic body, vesicle formation, and DNA ladder were confirmed in the light-irradiated. The caspase-3/7 activity an important factor in apoptosis, was 101.50 ± 14.24% when treated with 0.25 μg/ml methyl pheophorbide A in U937, and 91.32 ± 16.23% when treated with 1.00 μg/ml in SK-HEP-1 cells. The apoptotic phenomenon appeared more strongly when the methyl pheophorbide A concentration was increased with irradiating light. This study demonstrates the isolation, identification, and phototoxic activity of methyl pheophorbide A in perilla leaves. We expect that this study will be useful in the search for PS candidates using natural products.

中文翻译：

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紫苏叶光敏剂脱镁叶绿酸 A 的分离、鉴定及凋亡活性

光动力疗法 (PDT) 是一种副作用较少的有前途的癌症治疗方法，它通过光敏剂 (PS) 和光产生的活性氧消除靶组织中的肿瘤。在这项研究中，我们从紫苏叶中分离出甲基脱镁叶绿素 A，它在 U937 和 SK-HEP-1 细胞中诱导光动力细胞死亡。还评估了其作为开发新 PS 材料的潜力。脱镁叶绿酸甲酯是从紫苏叶甲醇提取物中分离出的深绿色卟啉化合物。脱镁叶绿素A处理后光照发生细胞凋亡，未光照无明显变化。在 U937 和 SK-HEP-1 细胞中，在光照射下都证实了凋亡小体、囊泡形成和 DNA 梯。caspase-3/7 活性是细胞凋亡的重要因素，在 U937 中用 0.25 μg/ml 脱镁叶绿酸 A 处理时为 101.50 ± 14.24%，在 SK-HEP-1 细胞中用 1.00 μg/ml 处理时为 91.32 ± 16.23%。当光照下甲基脱镁叶绿酸A浓度增加时，细胞凋亡现象更加强烈。本研究证明了紫苏叶中脱镁叶绿酸 A 的分离、鉴定和光毒性活性。我们预计这项研究将有助于寻找使用天然产物的 PS 候选者。紫苏叶甲基脱镁叶绿素 A 及其光毒活性。我们预计这项研究将有助于寻找使用天然产物的 PS 候选者。紫苏叶甲基脱镁叶绿素 A 及其光毒活性。我们预计这项研究将有助于寻找使用天然产物的 PS 候选者。