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Dual-Signaling Photoelectrochemical Biosensor Based on Biocatalysis-Induced Vulcanization of Bi2MoO6 Nanosheets
Analytical Chemistry ( IF 6.7 ) Pub Date : 2022-08-03 , DOI: 10.1021/acs.analchem.2c02848
Jianhui Xu 1 , Ruijin Zeng 1 , Lingting Huang 1 , Zhenli Qiu 2 , Dianping Tang 1
Affiliation  

A magnetic-assisted photoelectrochemical (PEC) and colorimetric (CL) dual-modal biosensing platform with high precision was established to monitor prostate-specific antigen (PSA) based on Bi2MoO6 nanosheets (BMO) by coupling the aptamer-guided hybridization chain reaction (HCR) with the hydrolysate-induced vulcanization reaction of Bi2MoO6 nanosheets. Upon addition of PSA, trigger DNA (tDNA) was released by the interaction between the target analyte and the aptamer and then further hybridized with anchor DNA (aDNA) conjugated on magnetic beads (MBs). The as-released tDNA initiated the target-assisted HCR in the presence of two alternating hairpin sequences (Bio-H1 and Bio-H2) to produce nicked long double-stranded DNA on the surface of MBs, where numerous alkaline phosphatase (ALP) enzymes could assemble with MBs through the biotin–avidin reaction, resulting in the hydrolysis of sodium thiophosphate (TP) to H2S. The as-produced H2S reacted with BMO to form vulcanized BMO (BMO-S), thus leading to obvious enhanced PEC performance under visible light with the color change from light yellow to brown. Having optimized the test conditions, the magnetic-assisted biosensing system holds a good quantitative diagnosis sensitivity area in a range of 5.0 pg mL–1–100 ng mL–1 with a calculated detection limit down to 3.5 pg mL–1. Meanwhile, a visual colorimetric assay on basis of the change in the color of the materials was also realized. Given the exceptional performance of the constructed biosensor, it may possess great promise as an advanced bioanalytical tool for practical applications.

中文翻译:

基于生物催化硫化Bi2MoO6纳米片的双信号光电化学生物传感器

建立了基于Bi 2 MoO 6纳米片(BMO)的高精度磁辅助光电化学(PEC)和比色(CL)双模态生物传感平台,通过耦合适体引导的杂交链来监测前列腺特异性抗原(PSA)。与水解产物引发的 Bi 2 MoO 6硫化反应 (HCR)纳米片。添加 PSA 后,触发 DNA (tDNA) 通过目标分析物和适体之间的相互作用释放,然后与结合在磁珠 (MB) 上的锚定 DNA (aDNA) 进一步杂交。释放的 tDNA 在存在两个交替发夹序列(Bio-H1 和 Bio-H2)的情况下启动靶标辅助 HCR,以在 MB 表面产生带切口的长双链 DNA,其中许多碱性磷酸酶 (ALP) 酶可以通过生物素-亲和素反应与 MBs 组装,导致硫代磷酸钠 (TP) 水解为 H 2 S。生成的 H 2S与BMO反应形成硫化BMO(BMO-S),从而导致可见光下PEC性能明显增强,颜色由浅黄色变为棕色。优化了测试条件后,磁辅助生物传感系统在 5.0 pg mL –1 –100 ng mL –1范围内具有良好的定量诊断灵敏度区域,计算出的检测限低至 3.5 pg mL –1。同时,还实现了基于材料颜色变化的视觉比色测定。鉴于构建的生物传感器的卓越性能,它可能具有作为实际应用的先进生物分析工具的巨大希望。
更新日期:2022-08-03
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