Herein, an autonomous driven DNA walker-based aptasensor is proposed for the detection of Ochratoxin A (OTA). A nanoscale metal-organic framework (MOF) is synthesized and used to load Mn²⁺, a cofactor for DNAzyme. The DNA walker and Mn²⁺@MOF are assembled on the Au surface, integrating the DNA walker and metal auxiliary ions. The proposed sensor avoids the addition of metal auxiliary ions of DNAzyme from the external environment, which achieves the fully autonomous driving of DNA walker and facilitates the on-site detection of OTA. In addition, the substrate strands are modified with a newly synthesized tetraferrocene signal marker, further achieving signal amplification. The sensor demonstrates a sensitivity of 0.289 pg/mL and is successfully applied to real food sample analysis.

在此，提出了一种基于自主驱动的 DNA 步行者的适体传感器，用于检测赭曲霉毒素 A (OTA)。纳米级金属有机框架 (MOF) 被合成并用于加载 Mn ²⁺，Mn 2+ 是 DNAzyme 的辅助因子。DNA walker 和Mn ²⁺ @MOF 组装在Au 表面，结合了DNA walker 和金属辅助离子。所提出的传感器避免了外界环境中DNAzyme金属辅助离子的加入，实现了DNA walker的全自动驾驶，便于OTA的现场检测。此外，底物链被新合成的四茂铁信号标记修饰，进一步实现了信号放大。该传感器的灵敏度为 0.289 pg/mL，并成功应用于实际食品样品分析。