Mucin 1(MUC1) is an effective marker of breast cancer, so it is of great significance to develop a simple, sensitive and highly selective MUC1 detection sensor. Herein, we constructed a label-free nanopore biosensor for rapid and highly sensitive detection of MUC1. The presence of MUC1 triggered the modification of the DNAzyme walking chain on the surface of Fe₃O₄ nanoparticles and separation from the aptamer. In the presence of Zn²⁺, DNAzyme catalyzed hydrolytic cleavage of the hairpin substrate at the scissile rA. The DNAzyme was divided into two fragments and ssDNA was released. ssDNA products from the hairpin substrate can generate a current blocking signal during α-hemolysin nanopore testing. The frequency of signature events showed a linear response toward the concentration of MUC1 in the range of 0.01 nM–100 nM. The sensing system also exhibited high selectivity against other protein and can be used for the detection of real sample.

粘蛋白1（MUC1）是乳腺癌的有效标志物，因此开发一种简单、灵敏、高选择性的MUC1检测传感器具有重要意义。在此，我们构建了一种无标记的纳米孔生物传感器，用于快速、高灵敏度地检测 MUC1。MUC1的存在引发了Fe ₃ O ₄纳米颗粒表面DNAzyme步链的修饰并与适配体分离。在存在 Zn ^{2+的情况下}，脱氧核糖核酸酶催化的发夹底物在易断裂的rA处水解切割。DNAzyme被分成两个片段并释放出ssDNA。来自发夹底物的 ssDNA 产物可以在 α-溶血素纳米孔测试期间产生电流阻断信号。特征事件的频率在 0.01 nM-100 nM 范围内显示出对 MUC1 浓度的线性响应。该传感系统还表现出对其他蛋白质的高选择性，可用于检测真实样品。