Polyethylene (PE) is one of the most widely used plastics. However, the chemical inertness, inefficient recycling, and random landfilling of PE waste have caused serious pollution to the natural environment. In this study, a series of laccase-mediator systems (LMS) were constructed by combination of two laccases from Botrytis aclada (BaLac) and Bacillus subtilis (BsLac) with three synthetic mediators (ABTS, HBT, and TEMPO) to oxidize LDPE films (UVPE) pretreated with high-temperature UV irradiation. Scanning electron microscopy showed aging phenomena such as etching, fragmentation, and cracking on the surface of the UVPE films after LMS incubation. The FTIR results showed that LMS-UVPE added new oxygen-containing functional groups such as -OH, -CO, and CC. High-temperature gel chromatography confirmed that the average reduction in weight-average molecular weight (Mw) was approximately 40% for the BaLac experimental group. GC-MS analysis showed the presence of oxygen-containing products, such as aldehydes, ketones, and alcohols, in the reaction mixture. To verify the oxidation process UVPE degradation by LMS, we inferred three possible pathways by combined analysis of the oxidation products of LMS on UVPE and model substrates oleic acid and squalene.

聚乙烯（PE）是应用最广泛的塑料之一。然而，PE废物的化学惰性、回收效率低、随意填埋等对自然环境造成了严重污染。在这项研究中，通过将来自Botrytis aclada (BaLac) 和枯草芽孢杆菌(BsLac) 的两种漆酶与三种合成介质 (ABTS、HBT 和 TEMPO)组合来氧化 LDPE 薄膜，构建了一系列漆酶介体系统 (LMS)。UVPE) 经高温紫外线照射预处理。扫描电镜显示LMS孵育后UVPE薄膜表面出现蚀刻、碎裂、开裂等老化现象。FTIR 结果表明 LMS-UVPE 添加了新的含氧官能团，如 -OH、-CO和 CC. 高温凝胶色谱证实，BaLac 实验组的重均分子量 (Mw) 平均降低了约 40%。GC-MS 分析表明反应混合物中存在含氧产物，例如醛、酮和醇。为了验证 LMS 对 UVPE 的氧化降解过程，我们通过结合分析 LMS 在 UVPE 和模型底物油酸和角鲨烯上的氧化产物，推断出三种可能的途径。