The widespread use of quinolones in humans and animals has become a major threat to public health. In this study, a simple, rapid, sensitive, and high throughput method based on automatic solid-phase extraction and isotope dilution ultra-performance liquid chromatography tandem mass spectrometry was described for the determination of trace quinolones in environmental water. The proposed automated solid-phase extraction method was initially optimized, and the optimum experimental conditions found were 1 L water sample with 0.5 g/L Na₂EDTA (pH 3) extracted and enriched by CNW Poly-Sery HLB cartridge at a flow rate of 50 mL/min and eluted by 8 mL of methanol. The linearity of the method ranged from 0.05 to 100 μg/L for 15 quinolones, with correlation coefficients ranging from 0.9993 to 0.9999. The limits of detection were in the low ng/L level, ranging from 0.005 to 0.051 ng/L. Finally, the optimized method was applied for determining trace levels of 15 quinolones in Wahaha pure water, tap water, river water, and seawater samples with good recoveries of 93 %–119 % and satisfactory relative standard deviations of 0.1 %–13.9 %. Fourteen quinolones were detected, and ofloxacin was the predominant congener in river water and seawater.

喹诺酮类药物在人类和动物中的广泛使用已成为对公共健康的主要威胁。在这项研究中，描述了一种基于自动固相萃取和同位素稀释超高效液相色谱串联质谱的简单、快速、灵敏和高通量的方法，用于测定环境水中的痕量喹诺酮类药物。对所提出的自动化固相萃取方法进行了初步优化，发现的最佳实验条件为 1 L 水样，含 0.5 g/L Na ₂EDTA (pH 3) 由 CNW Poly-Sery HLB 小柱以 50 mL/min 的流速萃取和富集，并由 8 mL 甲醇洗脱。该方法对 15 种喹诺酮类药物的线性范围为 0.05 至 100 μg/L，相关系数范围为 0.9993 至 0.9999。检测限处于低 ng/L 水平，范围为 0.005 至 0.051 ng/L。最后，将优化后的方法应用于娃哈哈纯水、自来水、河水和海水样品中痕量 15 种喹诺酮类药物的测定，回收率良好，回收率为 93%~119%，相对标准偏差为 0.1%~13.9%。共检测出 14 种喹诺酮类药物，其中氧氟沙星是河水和海水中的主要同源物。