Tumor-Targeting NIRF/MR Dual-Modal Molecular Imaging Probe for Surgery Navigation,Analytical Chemistry

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Tumor-Targeting NIRF/MR Dual-Modal Molecular Imaging Probe for Surgery Navigation
Analytical Chemistry ( IF 6.7 ) Pub Date : 2022-08-03 , DOI: 10.1021/acs.analchem.2c01790
Qiyi Li ₁ , Xin Xue ₂ , Jintao Wang ₁ , Yuting Ye ₃ , Jia Li ₃ , Yanwei Ren ₁ , Dandan Wang ₁ , Bing Liu ₁ , Yuyan Li ₁ , Li Zhao ₂ , Qingxiang Xu ₄

Affiliation

Multimodality imaging recognized as a promising monitoring strategy can serve the needs of accurate diagnosis and treatment of cancer by providing molecular and anatomic information about tumor sites. However, the probes based on multiple imaging modalities for surgery navigation remain limited due to poor biocompatibility and tumor targeting specificity. Herein, we present a small-molecule near-infrared fluorescence/magnetic resonance (NIRF/MR) imaging probe, Gd-NMC-3, covalently coupled with DCDSTCY and Gd-DOTA via butane diamine, for precise detection and intraoperative visualization. The in vitro and in vivo studies demonstrated that Gd-NMC-3 could be effectively accumulated in tumor sites as a bimodal imaging molecule exhibiting significant fluorescence accumulation and reasonable relaxation property in tumors with low cytotoxicity and good biocompatibility. Furthermore, Gd-NMC-3 was successfully applied to provide real-time visual navigation in LM3 orthotopic and subcutaneous tumor models to guide the resection of tumors. Importantly, no more fluorescence was observed in mice after operation, implying the total removal of tumor tissues. In conclusion, Gd-NMC-3 has great potential to be applied in the clinic based on its high resolution and sensitivity in tumor imaging.

中文翻译：

用于手术导航的肿瘤靶向 NIRF/MR 双模分子成像探头

多模态成像被认为是一种很有前途的监测策略，可以通过提供有关肿瘤部位的分子和解剖信息来满足准确诊断和治疗癌症的需求。然而，由于生物相容性差和肿瘤靶向特异性差，基于多种成像模式的手术导航探针仍然受到限制。在此，我们提出了一种小分子近红外荧光/磁共振 (NIRF/MR) 成像探针 Gd-NMC-3，通过丁二胺与 DCDSTCY 和 Gd-DOTA 共价偶联，用于精确检测和术中可视化。体外和体内_研究表明，Gd-NMC-3可以作为一种双峰成像分子在肿瘤部位有效积累，在肿瘤中表现出显着的荧光积累和合理的弛豫特性，具有低细胞毒性和良好的生物相容性。此外，Gd-NMC-3 成功应用于 LM3 原位和皮下肿瘤模型中提供实时视觉导航，以指导肿瘤切除。重要的是，手术后小鼠体内不再观察到荧光，这意味着肿瘤组织被完全切除。综上所述，Gd-NMC-3凭借其在肿瘤成像中的高分辨率和敏感性具有很大的临床应用潜力。

更新日期：2022-08-03

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