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Target-Modulated Hydrophobic Precipitation in Photocatalytic Nanochannels for Sensitive Detection of Alpha Fetoprotein
Analytical Chemistry ( IF 6.7 ) Pub Date : 2022-08-03 , DOI: 10.1021/acs.analchem.2c01937
Xia Zhao 1 , Jingwen Xu 1 , Zhenqing Dai 1 , Junli Guo 1 , Huijie Xu 1 , Junjian Zhao 1 , Yan-Yan Song 1 , Zhida Gao 1
Affiliation  

It is important to detect cancer biomarkers at an early stage of tumor development for the effective diagnosis and treatment of cancer. As a well-known probe for detecting superoxide (·O2) radicals, nitro blue tetrazolium (NBT) can rapidly react with ·O2 to form a hydrophobic formazan precipitate. In this study, by deliberately utilizing this reaction, Pt asymmetrically decorated on a TiO2 nanochannel membrane (Pt/TiNM) is explored to fabricate an electrochemical immunosensing platform with outstanding selectivity and ultrahigh sensitivity. Using NBT as the substrate, hydrophobic formazan precipitation induces a substantial block of ionic diffusion flux in nanochannels. Using alpha fetoprotein (AFP) as the target analyte, the established immunorecognition event was used to induce MoS2-Ab2 conjugates. Thanks to the excellent light-shielding ability of MoS2 nanosheets, the production of ·O2 radicals from the photocatalysis of Pt/TiNM is effectively depressed because of the attenuated arrival of light. The reduced formazan precipitation results in ionic transport changes in nanochannels, which in turn enables the selective recognition of AFP down to 2 ng mL–1. This target-modulated sensing strategy is also capable of sensing other immune targets, thus paving a new way for designing nanochannel-based sensing platforms.

中文翻译:

光催化纳米通道中的目标调节疏水沉淀用于甲胎蛋白的灵敏检测

在肿瘤发展的早期阶段检测癌症生物标志物对于有效诊断和治疗癌症非常重要。作为检测超氧化物(·O 2 )自由基的著名探针,硝基四唑蓝(NBT)可与·O 2 快速反应形成疏水性甲臜沉淀物。在这项研究中,通过有意利用该反应,Pt 不对称地装饰在 TiO 2探索纳米通道膜 (Pt/TiNM) 以制造具有出色选择性和超高灵敏度的电化学免疫传感平台。使用 NBT 作为底物,疏水性甲臜沉淀会在纳米通道中诱导大量离子扩散通量。使用甲胎蛋白 (AFP) 作为目标分析物,已建立的免疫识别事件用于诱导 MoS 2 -Ab 2缀合物。由于MoS 2纳米片优异的遮光能力,产生·O 2 -由于光的衰减到达,Pt/TiNM 光催化产生的自由基被有效抑制。减少的甲臜沉淀会导致纳米通道中的离子传输发生变化,从而使 AFP 选择性识别低至 2 ng mL –1。这种目标调制传感策略还能够传感其他免疫目标,从而为设计基于纳米通道的传感平台铺平了新的道路。
更新日期:2022-08-03
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