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Highly Efficient PTCA/Co3O4/CuO/S2O82– Ternary Electrochemiluminescence System Combined with a Portable Chip for Bioanalysis
ACS Sensors ( IF 8.2 ) Pub Date : 2022-08-02 , DOI: 10.1021/acssensors.2c00819 Xianzhen Song 1 , Lu Zhao 1 , Xiang Ren 1 , Tao Feng 1 , Hongmin Ma 1 , Dan Wu 1 , Yuyang Li 1 , Chuannan Luo 1 , Qin Wei 1
ACS Sensors ( IF 8.2 ) Pub Date : 2022-08-02 , DOI: 10.1021/acssensors.2c00819 Xianzhen Song 1 , Lu Zhao 1 , Xiang Ren 1 , Tao Feng 1 , Hongmin Ma 1 , Dan Wu 1 , Yuyang Li 1 , Chuannan Luo 1 , Qin Wei 1
Affiliation
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Herein, we reported an efficient electrochemiluminescence (ECL) biosensor chip for sensitive detection of neuron-specific enolase (NSE). First, 3,4,9,10-perylenetetracarboxylic acid with good luminescence characteristics was used as a luminophore to obtain a stable ECL signal. Subsequently, hollow porous Co3O4/CuO concave polyhedron nanocages (CPNCs) were designed as co-reaction promoters to amplify the luminescence signals for highly sensitive trace detection of NSE. In brief, the rapid cyclic conversion of Co3+/Co2+ and Cu2+/Cu+ redox pairs could continuously catalyze the reduction of persulfate (S2O82–), thus providing a large number of essential active intermediates (SO4•–) for ECL emission. Meanwhile, the unique structure of Co3O4/CuO CPNCs possessed a large specific surface area, which greatly improved its catalytic efficiency. Third, NKFRGKYKC was developed as an affinity ligand for specific antibody fixation, which improved incubation efficiency and protected bioactivity of antibodies. Finally, we independently designed a microchip and applied it for ECL detection to improve the practical application ability of the sensor. The developed biosensor exhibited good sensitivity with a wide linear range (10 fg/mL to 100 ng/mL) and a low detection limit (3.42 fg/mL), which played an active role in the clinical application of sensing analysis.
中文翻译：
高效 PTCA/Co3O4/CuO/S2O82- 三元电化学发光系统结合便携式芯片进行生物分析
在此，我们报道了一种高效的电化学发光 (ECL) 生物传感器芯片，用于灵敏检测神经元特异性烯醇化酶 (NSE)。首先，使用具有良好发光特性的3,4,9,10-苝四甲酸作为发光体以获得稳定的ECL信号。随后，中空多孔 Co 3 O 4 /CuO 凹面多面体纳米笼 (CPNCs) 被设计为共反应促进剂，以放大发光信号，用于 NSE 的高灵敏度痕量检测。简而言之，Co 3+ /Co 2+和 Cu 2+ /Cu +氧化还原对的快速循环转化可以连续催化还原过硫酸盐 (S 2 O 8 2–), 从而为 ECL 发射提供大量必需的活性中间体 (SO 4 •– )。同时，Co 3 O 4 /CuO CPNCs独特的结构具有较大的比表面积，大大提高了催化效率。第三，NKFRGKYKC被开发为特异性抗体固定的亲和配体，提高了孵育效率并保护了抗体的生物活性。最后，我们自主设计了微芯片并将其应用于ECL检测，提高了传感器的实际应用能力。所开发的生物传感器具有良好的灵敏度、宽线性范围（10 fg/mL 至 100 ng/mL）和低检测限（3.42 fg/mL），在传感分析的临床应用中发挥了积极作用。
更新日期：2022-08-02
中文翻译：
高效 PTCA/Co3O4/CuO/S2O82- 三元电化学发光系统结合便携式芯片进行生物分析
在此，我们报道了一种高效的电化学发光 (ECL) 生物传感器芯片，用于灵敏检测神经元特异性烯醇化酶 (NSE)。首先，使用具有良好发光特性的3,4,9,10-苝四甲酸作为发光体以获得稳定的ECL信号。随后，中空多孔 Co 3 O 4 /CuO 凹面多面体纳米笼 (CPNCs) 被设计为共反应促进剂，以放大发光信号，用于 NSE 的高灵敏度痕量检测。简而言之，Co 3+ /Co 2+和 Cu 2+ /Cu +氧化还原对的快速循环转化可以连续催化还原过硫酸盐 (S 2 O 8 2–), 从而为 ECL 发射提供大量必需的活性中间体 (SO 4 •– )。同时，Co 3 O 4 /CuO CPNCs独特的结构具有较大的比表面积，大大提高了催化效率。第三，NKFRGKYKC被开发为特异性抗体固定的亲和配体，提高了孵育效率并保护了抗体的生物活性。最后，我们自主设计了微芯片并将其应用于ECL检测，提高了传感器的实际应用能力。所开发的生物传感器具有良好的灵敏度、宽线性范围（10 fg/mL 至 100 ng/mL）和低检测限（3.42 fg/mL），在传感分析的临床应用中发挥了积极作用。
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